血液肿瘤患者AHSCT前后外周血淋巴细胞亚群及细胞周期变化

发布时间：2018-01-19 06:07

本文关键词： 自体干细胞移植免疫重建淋巴细胞稳态增殖细胞周期　出处：《汕头大学》2011年硕士论文　论文类型：学位论文

【摘要】：【目的】 1、分析自体造血干细胞移植(Autologous hematopoietic stem cell transplantation ,AHSCT)前外周血淋巴细胞亚群及细胞周期的情况。 2、分析AHSCT后外周血淋巴细胞亚群及细胞周期变化的规律,初步了解血液肿瘤AHSCT后外周血淋巴细胞的免疫重建。【方法】 1、应用流式细胞术(Flow cytometry,FCM)分析21例血液肿瘤患者AHSCT前后淋巴细胞亚群变化。 2、应用FCM测定15例血液肿瘤患者AHSCT前后外周血淋巴细胞细胞周期变化。【结果】 1、CD3~+CD4~+细胞在AHSCT前低于正常。淋巴细胞总数在AHSCT后1月明显低于正常,在AHSCT后3月恢复正常,其各淋巴细胞亚群恢复时间不同,其中自然杀伤(Natural killer,NK)细胞、B细胞在3个月恢复,T细胞AHSCT后9个月恢复正常,其中主要为CD3~+CD8~+细胞,CD3~+CD8~+细胞在AHSCT后1月有所下降,在3个月时恢复并高于正常,至9个月时逐渐下降至正常,而CD3~+CD4~+细胞在AHSCT后9个月仍然远低于正常,其恢复正常需要更长时间。 2、外周血淋巴细胞的DNA合成期细胞比率(S-phage fraction,SPF)、细胞凋亡率(Apoptosis,APO)在AHSCT前正常:AHSCT后SPF明显高于正常,在AHSCT后9个月时恢复正常。Apo在AHSCT后1月时明显高于移植前,但其迅速下降,在3个月时稍高于正常,其后降至正常。【结论】 1、血液肿瘤患者AHSCT前存在淋巴细胞亚群异常,AHSCT后淋巴细胞总数、NK细胞、B细胞在AHSCT后早期较快恢复,T细胞亚群中CD3~+CD8~+细胞恢复较早,而CD3~+CD4~+细胞恢复缓慢。 2、血液肿瘤患者AHSCT前外周血淋巴细胞细胞周期分布正常,AHSCT后淋巴细胞APO和SPF均明显高于正常,其中APO在AHSCT后6月恢复正常,SPF在AHSCT后9月恢复正常。
[Abstract]:[Objective]
1, the peripheral blood lymphocyte subsets and cell cycle of autologous hematopoietic stem cell transplantation (Autologous hematopoietic stem cell transplantation, AHSCT) were analyzed.
2, the changes of lymphocyte subsets and cell cycle in peripheral blood after AHSCT were analyzed, and the immune reconstruction of peripheral blood lymphocytes in blood after AHSCT was preliminarily understood.
[method]
1, the changes of lymphocyte subsets before and after AHSCT in 21 blood tumor patients were analyzed by Flow cytometry (FCM).
2, the cell cycle changes in peripheral blood lymphocytes of 15 patients with hematologic tumor were measured by FCM.
[results]
1, CD3~+CD4~+ cells was lower than normal before AHSCT. The total number of lymphocytes in AHSCT after January was significantly lower than the normal, returned to normal at AHSCT after March, the lymphocyte subsets with different recovery time, the natural killer (Natural killer NK) cells, B cells in the 3 months of recovery, T cell AHSCT 9 months after recovery normally, including CD3~+CD8~+ cells, CD3~+CD8~+ cells in January AHSCT decreased after 3 months in recovery and higher than normal at 9 months decreased to normal, while CD3~+CD4~+ cells in AHSCT after 9 months is still far below normal, the recovery will take longer.
2, peripheral blood lymphocyte DNA synthesis cell ratio (S-phage fraction, SPF), cell apoptosis rate (Apoptosis, APO) in AHSCT normal before: SPF was significantly higher than that of normal AHSCT, AHSCT in the 9 months after the restoration of normal.Apo was significantly higher than that in AHSCT after January before transplantation, but it decreased rapidly. In 3 months, slightly higher than the normal, then returned to normal.
[Conclusion]
1, blood tumor patients had abnormal lymphocyte subsets before AHSCT. After AHSCT, the total number of lymphocytes, NK cells and B cells recovered rapidly at the early stage after AHSCT. The CD3~+CD8~+ cells in T cell subgroup recovered earlier, while CD3~+CD4~+ cells recovered slowly.
2, the cell cycle distribution of peripheral blood lymphocytes was normal in patients with hematological malignancies before AHSCT. After AHSCT, APO and SPF in lymphocytes were significantly higher than those in normal ones. APO returned to normal after AHSCT in June, and SPF returned to normal in September.

【学位授予单位】：汕头大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R392

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