玄参对脾虚水湿不化大鼠模型的影响及其肝脏代谢组学分析

发布时间：2018-01-24 18:34

本文关键词： 玄参脾虚水湿不化代谢组学生物标志物代谢通路黄嘌呤磷脂酰乙醇胺　出处：《中国实验方剂学杂志》2017年20期 　论文类型：期刊论文

【摘要】：目的:探索玄参对脾虚水湿不化大鼠模型的影响,并利用代谢组学方法对生物标志物和代谢通路进行分析。方法:高脂低蛋白饲料喂养加尾部负重游泳的方法建立脾虚水湿不化大鼠模型,玄参水煎液高、低剂量给药2周后检测各给药组肝脏指数、血清蛋白、血脂、胃肠功能和水负荷的变化情况,给药剂量分别为1.35,5.40 g·kg-1。利用UPLC-Q-TOF-MS检测肝组织中内源性小分子代谢物,电喷雾正离子模式(ESI+)和负离子模式(ESI-)对样品进行检测,m/z 100~1 500;利用主成分分析(PCA)和偏最小二乘法-判别分析(PLS-DA)比较空白组、模型组,玄参高、低剂量组内源性小分子代谢物的变化情况;对差异代谢物进行京都基因与基因组百科全书(KEGG)代谢通路分析。结果:与模型组比较,玄参高剂量组的胃排空率和D-木糖排泄率显著升高;玄参低剂量组能升高血清白蛋白、总蛋白含量;玄参高剂量组能降低总胆固醇、低密度脂蛋白胆固醇含量和水负荷指数。代谢组学方法共鉴定出21个生物标志物,找到7条主要代谢通路,主要涉及胰岛素分泌、甘油磷脂代谢、嘌呤代谢、胆汁酸分泌、磷脂酶D信号通路、糖基磷脂酰肌醇锚的合成、内源性大麻素的生物合成等。结论:玄参对脾虚水湿不化模型大鼠的影响与改善消化功能、抑制糖代谢、改善血脂代谢等有关。
[Abstract]:Objective: to explore the effect of Xuanshen on the rat model of spleen deficiency and dampness. The biomarkers and metabolic pathways were analyzed by the method of metabolomics. Methods: high fat and low protein feed plus tail weight bearing swimming method was used to establish the model of spleen deficiency water dampness in rats, and the decoction of Radix Sophora sinensis was high. The changes of liver index, serum protein, blood lipid, gastrointestinal function and water load in each group were measured 2 weeks after low dose administration, the dosage was 1.35 respectively. 5.40g 路kg-1.The endogenous small molecule metabolites in liver tissue were detected by UPLC-Q-TOF-MS. Electrospray positive ion mode (ESI) and negative ion mode (ESI) were used to detect the samples. The changes of endogenous small molecule metabolites in blank group, model group, high and low dose group were compared by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DAA). Results: compared with the model group, the gastric emptying rate and the D-xylose excretion rate of the high dose group were significantly higher than those of the model group. The contents of serum albumin and total protein were increased in the low dose group. High dose group could reduce total cholesterol, low density lipoprotein cholesterol content and water load index. A total of 21 biomarkers were identified and 7 main metabolic pathways were found. Mainly involved in insulin secretion, glycerol phospholipid metabolism, purine metabolism, bile acid secretion, phospholipase D signaling pathway, glycosylphosphatidylinositol anchor synthesis. Conclusion: the effect of Radix Xuanshen on spleen deficiency model rats is related to improving digestive function, inhibiting glucose metabolism and improving metabolism of blood lipids.
【作者单位】：黑龙江中医药大学中医药研究院黑龙江省高等院校中药药性理论创新团队药物安全性评价中心;
【基金】：国家重点基础研究发展计划(973计划)项目(2013CB531804)
【分类号】：R285.5;R-332
【正文快照】： [网络出版地址]http://kns.cnki.net/kcms/detail/11.3495.r.20170731.1054.080.html[网络出版时间]2017-07-31 10:54中医所论述的“脾”是以消化系统为主的多系统、多器官的功能综合单位。“脾主运化”是脾的主要生理功能。脾气虚则气化不行,湿邪积滞,气不化湿,即水湿不化,临

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