大鼠复合型放射性下颌骨骨坏死动物模型建立及观察

发布时间：2018-01-24 22:29

本文关键词： 骨坏死放射性颌模型动物纤维化骨组织形态学分析　出处：《中华口腔医学研究杂志(电子版)》2016年01期 　论文类型：期刊论文

【摘要】：目的依据颌骨放射性骨坏死(ORNJ)新的临床诊断标准,探讨建立可模拟ORNJ不同临床分期的复合型动物模型的方法。方法健康SD雄性大鼠36只,随机分为6组:a1、b1、c1为实验组,a0、b0、c0组为对照组;根据生物学等效公式,实验组采用电子直线加速器对大鼠左侧下颌区进行照射,7Gy/次,1次/d,照射5 d;照射后6周,处死a1、a0组大鼠,并拔除b1、b0、c1、c0组大鼠左侧下颌磨牙;照射12、18周后,分别处死b1、b0组大鼠和c1、c0组大鼠并取下颌骨标本。对所有标本进行组织学观察和micro-CT扫描与骨组织形态学分析。使用SPSS 18.0软件包对数据进行统计学分析。结果实验组照射区可见a1组门齿生长障碍、b1组死骨暴露、c1组咬合错乱,化脓性感染与病理性骨折等症状;对照组无症状。组织学观察可见,实验组照射区骨髓腔与牙周膜内,炎性细胞浸润、纤维组织增生,b1、c1组还可见游离死骨;实验组门齿牙根与皮质骨渐进性破坏,c1组部分大鼠门齿牙根结构消失,周围皮质骨不连续;实验组空白骨陷窝增多,骨陷窝内骨细胞减少。micro-CT结果显示:相对骨体积(BV/TV):a1(0.401±0.053)、a0(0.615±0.037)、b1(0.309±0.041)、b0(0.717±0.040)、c1(0.200±0.026)、c0(0.724±0.041),实验组相对骨体积(BV/TV)均低于对照组;实验组间BV/TV值相比,a1b1c1,Pa1、b1=0.012、Pb1、c1=0.034、Pc1、a1=0.004,差异有统计学意义(P0.05)。结论建立了一种能够模拟出轻度无症状(a1)、中度死骨暴露(b1)与重度联合症状(c1)三种不同ORNJ分型的复合型动物模型,为针对性研究ORNJ治疗方法提供了实验平台。
[Abstract]:Objective according to the new clinical diagnostic criteria of osteonecrosis of jaws (ORNJ), to explore the method of establishing compound animal model which can simulate different clinical stages of ORNJ. Methods 36 healthy male SD rats were used. The rats were randomly divided into 6 groups: the control group was the control group (n = 6). According to the biological equivalent formula, the experimental group irradiated the left mandibular region of rats with electron linear accelerator for 7 Gy / once a day for 5 days. Six weeks after irradiation, the rats in group A were killed and the left mandibular molars of group B 1 were extracted. After 12 weeks of irradiation, the rats in group B _ (1) were killed, and the rats in group C _ (1) were killed. The mandibular specimens were taken from c0 group rats. Histological observation, micro-CT scanning and bone histomorphology analysis were performed on all the specimens. SPSS was used. The data were analyzed by 18.0 software package. Results in the irradiated area of experimental group, the growth disorder of gentgear was found in group A1. Group B 1 was exposed to dead bone, and the symptoms of occlusal dislocation, suppurative infection and pathological fracture were found in group C1. The control group was asymptomatic. Histological observation showed that free dead bone could be seen in experimental group (n = 10). In experimental group, inflammatory cells were infiltrated in the medullary cavity and periodontal ligament in the irradiated area, and free dead bone was found in group B _ 1C _ 1 of fibrous tissue proliferation. The incisor root and cortical bone were destroyed gradually in the experimental group. Some of the incisor root structure disappeared and the cortical bone was discontinuous in the experimental group. In the experimental group, the number of empty bone lacunae increased, and bone cells decreased. Micro-CT results showed that the relative volume of bone was 0.401 卤0.053. A 0. 615 卤0. 037 B1 + 0. 309 卤0. 041 and 0. 717 卤0. 040 ~ 0. 00.200 卤0. 026). The relative bone volume (BV / TVV) of the experimental group was lower than that of the control group (0. 724 卤0. 041 卤0. 024 卤0. 041). The BV/TV value of the experimental group was 0.012 Pb1 / c1 ~ (1) and 0.034 ~ (4) Pc1C ~ (1 +) ~ (1) ~ 0. 004, compared with that of the control group. The difference was statistically significant (P 0.05). Conclusion A kind of mild asymptomatic analogue was established. The combined animal models of moderate dead bone exposure (b1) and severe syndromes (c1) were divided into three different types of ORNJ, which provided an experimental platform for the study of ORNJ therapy.
【作者单位】：第四军医大学口腔医院口腔颌面外科;
【基金】：国家自然科学基金(81202150/H2201) 陕西省社会发展科技攻关项目(2015SF140)
【分类号】：R730.55;R782;R-332
【正文快照】： 放射治疗是头颈部恶性肿瘤治疗的有效手段,但可造成各种并发症,其中以颌骨放射性骨坏死(osteoradionecrosis of jaws,ORNJ)最为严重,其发病率约为8.2%~22%[1]。目前,ORNJ的诊断尚无统一标准,普遍认为ORNJ是在无肿瘤复发或残余的情况下,受照射区域骨组织暴露于皮肤或黏膜外,超

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