三步序贯法对哮喘大鼠模型激素撤减中转化生长因子β1及胶原表达的影响

发布时间：2018-01-27 06:25

  本文关键词： 三步序贯法 哮喘 激素撤减 转化生长因子-β 胶原　出处：《中华中医药杂志》2016年07期 　论文类型：期刊论文

【摘要】：目的:观察三步序贯法对哮喘模型大鼠激素撤减过程中转化生长因子-β(T G F-β)及胶原(Collagen)表达的影响。方法:将150只雄性SD大鼠随机分为正常对照组、气道重塑组、地塞米松组、普米克令舒组及三步序贯组。模型建立方法:以卵蛋白100mg加氢氧化铝100mg致敏,1%卵蛋白激发,自激发之日开始,于激发前30min给予地塞米松0.5mg/kg腹腔注射,连续2周,从第3周开始,按每周0.1mg/kg速度递减地塞米松用量,至第7周地塞米松全部撤除。三步序贯组在地塞米松干预基础上,于实验第15-28天(地塞米松撤前)给予第1步方颗粒灌胃,第29-63天(地塞米松撤中)给予第2步方颗粒灌胃,第64-77天(地塞米松撤后)给予第3步方颗粒灌胃,每日1次。各组于实验流程第28、63、77天末次激发2h后取左肺组织行免疫组化检测,比较各组TGF-β1、CollagenⅠ、CollagenⅢ蛋白表达变化。结果:在撤减过程中,与正常对照组比较,气道重塑组和地塞米松组CollagenⅠ蛋白表达升高(P0.05),TGF-β1、CollagenⅢ均显著升高(P0.01);与地塞米松组比较,三步序贯组TGF-β1、CollagenⅠ显著降低(P0.01,P0.05),CollagenⅢ在撤减中后期降低(P0.05)。结论:三步序贯法对哮喘模型大鼠撤减激素过程中气道重塑的阻抑作用可能是通过降低TGF-β1,调控其介导的信号通路,进而减少CollagenⅠ和CollagenⅢ沉积来实现的。
[Abstract]:Objective: to observe the effect of three-step sequential method on transforming growth factor- 尾 (TGF- 尾) and collagen collagen during hormone withdrawal in asthmatic rats. Methods: 150 male SD rats were randomly divided into normal control group. Airway remodeling group, dexamethasone group, Pulmicort group and three-step sequential group. Methods: ovalbumin 100 mg + aluminum hydroxide 100 mg sensitized with 1% ovalbumin was induced from the day of stimulation. Dexamethasone 0.5 mg / kg was injected intraperitoneally 30 minutes before challenge for 2 weeks. From the third week, the dosage of dexamethasone was decreased at the rate of 0.1 mg / kg per week. By week 7, dexamethasone was completely removed. On the basis of dexamethasone intervention, the three-step sequential group was given intragastric infusion of Dexamethasone granule on the 15-28 days (before dexamethasone withdrawal). In the 29-63 days (dexamethasone withdrawal), the second step granule was given intragastrically, and the 64-77 days (dexamethasone withdrawal) was given to the third step granule once a day. The left lung tissues were collected for immunohistochemical examination 2 hours after the last stimulation on the 77th day, and TGF- 尾 _ 1 Collagen 鈪，

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