冠心病复合高血脂状态病证结合动物模型的建立与评价

发布时间：2018-01-29 00:18

本文关键词： 冠心病高脂病证结合动物模型气虚血瘀痰浊　出处：《中华中医药杂志》2016年05期 　论文类型：期刊论文

【摘要】：目的:从疾病和证候两个层面对冠心病复合高血脂状态的大鼠模型进行证候属性的多时间点动态评价。方法:模拟临床冠心病发病特点,采用高脂饮食联合左冠脉结扎术制备模型。术后7、14、28d,动态检测血脂、心电图、超声心动等疾病指标,旷场实验、应激状态下呼吸频率、足底RGB值、体质量、体质量增量、BMI、24h进食量等宏观体征进行证候判定。结果:冠心病复合高血脂状态模型在术后7d心电图检测呈现6-8个病理性Q波纳入研究,术后7、14、28d,TC、TG、LDL-C均显著升高(P0.05,P0.01)。超声心动显示EF值、FS值均显著降低,LVID;d、LVID;s显著升高(P0.01),旷场3min自主运动距离显著下降(P0.01),应激状态下呼吸频率显著升高(P0.05,P0.01);足底RGB均值和R值显著降低(P0.05,P0.01);体质量和进食量先降后升,至术后28d体质量显著升高(P0.05,P0.01),自术后14d始出现体质量增量和BMI的显著升高(P0.05,P0.01)。结论:冠心病复合高血脂状态大鼠模型在术后7-14d属于气虚血瘀证,14-28d过渡为气虚血瘀兼有痰浊证。
[Abstract]:Objective: to evaluate the syndromes of coronary heart disease (CHD) combined with hyperlipidemia in a rat model from two levels of disease and syndromes. Methods: to simulate the clinical characteristics of coronary heart disease (CHD). High fat diet combined with left coronary ligation was used to establish the model. Blood lipid, electrocardiogram, echocardiography and other disease indexes, open field experiment and respiratory frequency under stress were dynamically detected at 7 ~ 14 ~ 28 days after operation. The RGB of plantar, body mass and body mass were increased. Results: coronary heart disease combined with hyperlipidemia state model showed 6-8 pathological Q waves at 7 days after operation. The LDL-C levels of TGG in TCU were significantly higher than those of P0.05 and P0.01.The EF and FS values were significantly decreased by echocardiography. Dl LVID; S significantly increased P0.01A, decreased the distance of spontaneous exercise for 3 min in open field, and increased the respiratory frequency of P0.05 and P0.01in stress state. The mean value of RGB and R value of plantar decreased significantly (P 0.05 P 0.01). The body mass and food intake decreased first and then increased, and then increased to 28 days after the operation, the body mass increased significantly (P 0.05) and the BMI increased significantly (P 0.05) from the 14th day after operation. Conclusion: the rat model of coronary heart disease combined with hyperlipidemia belongs to Qi deficiency and blood stasis syndrome from 14 to 28 days after operation.
【作者单位】：北京中医药大学基础医学院;北京中医药大学中药学院;北京中医药大学中药现代研究中心;北京中医药大学科研实验中心;北京市心肺血管疾病研究所;
【基金】：国家自然科学基金项目(No.81470191,No.81530100,No.81473456) 北京中医药大学在读研究生项目(No.2015-JYB-XS046)~~
【分类号】：R259;R-332
【正文快照】： 冠心病患者证候分布特点以血瘀为最多,其次为气虚和痰浊[1-3]。本课题组前期研究模拟冠心病核心病理机制——心肌缺血,通过结扎大鼠左冠状动脉成功制备动物模型,经宏观表征与理化指标的动态观察与评价,判定其术后7-28d符合冠心病气虚血瘀证[4]。而临床冠心病病机复杂,常伴有高

【相似文献】