迷迭香提取物对小鼠急性酒精肝模型保护作用研究

发布时间：2018-01-30 00:54

本文关键词： 迷迭香提取物急性酒精肝氧化损伤脂代谢炎症　出处：《天然产物研究与开发》2017年02期 　论文类型：期刊论文

【摘要】：研究迷迭香提取物(Rosemary Extract,RE)对小鼠急性酒精肝损伤的保护作用,并从酒精代谢、脂代谢、抗氧化和抗炎几个方面探讨其作用机制。将小鼠随机分为空白对照组(Control)、模型组(Model)、欣立得组(Metadoxine Capsules,MC,200 mg/kg·d),RE剂量组80、160、400 mg/kg·d,给药30 d后建立小鼠急性酒精肝损伤模型,检测血清ALT、AST、TG、TNF-α、IL-6、IL-10浓度,肝脏MDA、SOD、GSH-Px、ADH活性,qRT-PCR检测肝脏脂肪酸合成酶(FAS)、脂肪分化相关蛋白(ADRP)、细胞色素P450 2E1(CYP2E1)、过氧化物酶体增值物激活α受体(PPARα)和半胱氨酸天冬氨酸蛋白酶3(caspase3)mRNA的表达,HE染色观察肝脏组织病理变化。RE组小鼠血清ADH活性升高,ALT、AST和TG含量降低,醒酒时间缩短,肝脏组织脂肪变性减轻,细胞凋亡相关基因Caspase3表达降低,保护机制研究发现RE能下调FAS和ADRP基因表达,减少肝脏脂肪合成;提高抗氧化损伤酶SOD、GSH-Px活性,下调酒精代谢中ROS合成基因CYP2E1和上调抗氧化损伤和炎症基因PPARα表达,使MDA浓度降低,减轻肝脏氧化损伤;降低炎性因子TNF-α和IL-6浓度,提高抗炎因子IL-10浓度。实验结果表明RE对小鼠急性酒精肝损伤具有保护作用,其作用机制可能与抗氧化、抗炎和脂肪代谢调节有关。
[Abstract]:To study the protective effect of Rosemary ExtractRE) on acute alcoholic liver injury in mice from alcohol metabolism and lipid metabolism. To explore the mechanism of anti-oxidation and anti-inflammation, mice were randomly divided into blank control group (control group) and model group (model group). Metadoxine Capsulesus, 200 mg/kg 路d, RE group, 80,160,400 mg/kg 路d. A model of acute alcoholic liver injury was established in mice 30 days after administration. The concentration of IL-10 in serum of TGN TNF- 伪 and MDA-SOD in liver was detected. Liver fatty acid synthase (FASP) and adipose differentiation related protein (ADRP) were detected by qRT-PCR. Cytochrome P450 2E1 (CYP2E1). Peroxisome value-added activated the expression of PPAR 伪 and caspase3 mRNA of cysteine aspartate protease 3. The activity of serum ADH was increased and the contents of alt AST and TG were decreased, the time of soaking up was shortened, and the steatosis of liver tissue was reduced in the group of HE staining. The expression of apoptosis-related gene Caspase3 was decreased. The protective mechanism showed that RE could down-regulate the expression of FAS and ADRP genes and reduce hepatic fat synthesis. The activity of GSH-Px was increased, the ROS synthesis gene CYP2E1 and the expression of antioxidant injury and inflammatory gene PPAR 伪 were down-regulated in alcohol metabolism. The concentration of MDA was decreased and the oxidative damage of liver was alleviated. The experimental results showed that RE has protective effect on acute alcoholic liver injury in mice, and its mechanism may be related to antioxidation. Anti-inflammatory and fat metabolism regulation.
【作者单位】：昆明医科大学生物医学工程研究中心;昆明医科大学药学院暨云南省天然药物药理重点实验室;
【基金】：云南省南药研究协同创新中心项目(NY2014002)
【分类号】：R285.5;R-332
【正文快照】： 酒精性肝病(alcoholic liver disease,ALD)是由于过度饮酒导致的肝损伤,也是引发肝脏疾病的重要原因。人体长期饮酒或短期大量饮酒会引起酒精性肝细胞损伤,导致肝细胞发生脂肪变性、炎症和坏死等[1]病变,其发病机制与酒精代谢引发的氧化应激、细胞因子释放、线粒体损伤、肝细

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