附骨疽动物模型的制备及验证

发布时间：2018-01-31 12:34

本文关键词： 附骨疽动物模型冰敷金黄色葡萄球菌　出处：《中华中医药杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:改进附骨疽动物模型的制备方法并完善模型验证指标。方法:将新西兰兔随机分为空白对照组、附骨疽模型组与细菌感染模型组,附骨疽模型组动物以冰敷(10min/d)15d,采用金黄色葡萄球菌局部感染方法造模,细菌感染模型组以金黄色葡萄球菌局部感染方法造模。以血液流变学指标、血液炎性反应指标、病理组织学指标验证模型。结果:附骨疽模型组与空白对照组比较,血液黏度增高(P0.01),附骨疽模型组与细菌感染模型组比较,白细胞计数、超敏C反应蛋白显著升高(P0.01);组织病理学检查显示:附骨疽模型组坏死组织范围较大,肉芽组织显著增多。结论:局部冰敷结合细菌感染的方法可用于附骨疽模型的制备,为附骨疽病因探究及中药疗效评价提供方法与依据。
[Abstract]:Objective: to improve the preparation method of the animal model of osteoanthrax and to improve the verification index of the model. Methods: new Zealand rabbits were randomly divided into three groups: the control group, the model group and the bacterial infection model group. The rats in the model group were treated with ice for 10 min / d for 15 days and staphylococcus aureus local infection was used to make the model. The bacterial infection model group was made by the method of local infection of Staphylococcus aureus, the hemorheology index and the blood inflammatory reaction index. Results: compared with the blank control group, the blood viscosity of the model group was higher than that of the control group (P 0.01), and the leukocyte count of the model group was higher than that of the model group of bacterial infection. Hypersensitive C-reactive protein significantly increased P0.01C; Histopathological examination showed that the necrotic tissue was larger and the granulation tissue was significantly increased in the osteoanthrax model group. Conclusion: the method of local ice compress combined with bacterial infection can be used for the preparation of osteoanthrax model. To provide the method and basis for exploring the etiology of bone gangrene and evaluating the curative effect of traditional Chinese medicine.
【作者单位】：浙江省中医药研究院中药研究中心;浙江省立同德医院骨科;
【基金】：浙江省实验动物科技计划(No.2014C37033) 浙江省医药卫生科技计划(No.2014KYA029,No.2016RCB003) 浙江省中医药科技计划(No.2015ZA019)~~
【分类号】：R-332;R274.9
【正文快照】： 附骨疽,现代医学称之为慢性骨髓炎,病程长,反复发作,是骨科复杂病种[1]。基于其发病率呈上升趋势,临床治疗中尚存在骨愈合时间长、骨不连并发症高发、愈后患者运动功能受损严重等现实问题[2-4]。笔者目前尚未见关于附骨疽动物模型的研究报道。建立附骨疽动物模型,有利于进一步

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