2011～2014年中国福建龙岩市Echo 30型病毒分子流行病学特征分析

发布时间：2018-01-31 11:39

  本文关键词： Echo 型病毒 病毒性脑炎 VP 分子流行病学　出处：《病毒学报》2016年06期 　论文类型：期刊论文

【摘要】：了解中国福建省龙岩市2011~2014年病毒性脑炎散发病例中Echo 30分子流行病学特征。收集病毒性脑炎或中枢神经系统感染脑脊液标本,细胞培养分离病毒,RT-PCR扩增VP1基因序列全长以鉴定Echo 30血清型及遗传特征分析。2011~2014年共从608例监测病例中分离到168株肠道病毒,其中60株为Echo 30;60例Echo 30相关病例分析显示,Echo 30流行高峰为6~8月;波及年龄范围广,以小于10岁高发(65%);临床症状以发热、头痛和呕吐为主,脑脊液清,细胞和蛋白含量检测多增加。VP1区分析显示,2011~2014年龙岩流行株均属于h基因型,但有两个传播链共同循环;与2011年导致福建省病毒性脑炎暴发毒株高度同源,但2014年龙岩株均在VP1蛋白I 120V出现新氨基酸变异。福建省2011年暴发流行株仍流行于龙岩市,且两个传播链病毒仍共同循环,但2014年病毒株出现了新的变异,持续监测将有利于早期发现病毒变异积累和评估疾病流行的风险。
[Abstract]:To investigate the molecular epidemiological characteristics of Echo 30 in sporadic cases of viral encephalitis from 2011 to 2014 in Longyan City, Fujian Province, China. The cerebrospinal fluid (CSF) samples of viral encephalitis or central nervous system infection were collected. The virus was isolated by cell culture. Amplification of full length VP1 Gene sequence by RT-PCR to identify Echo. Analysis of 30 serotypes and genetic characteristics. 168 strains of enterovirus were isolated from 608 surveillance cases from 2011 to 2014. Among them, 60 strains were Echo 30; The analysis of 60 Echo 30 related cases showed that the peak of Echo 30 epidemic was from 6 to August. The affected age was wide, with a high incidence of 65% less than 10 years old; The clinical symptoms were fever, headache and vomiting. The detection of cerebrospinal fluid (CSF), cell and protein increased. The analysis of VP1 region showed that Longyan epidemic strain belonged to h genotype from 2011 to 2014. However, there are two transmission chains in common circulation; It is highly homologous to the virus encephalitis outbreak in Fujian province in 2011. In 2014, the new amino acid variation of Longyan strain was found in VP1 protein I120V. the outbreak in Fujian Province in 2011 was still prevalent in Longyan City, and the two transmission chain viruses were still circulating together. In 2014, however, a new mutation appeared in the virus strain, and continuous monitoring will facilitate early detection of the accumulation of variation and assessment of the risk of disease prevalence.
【作者单位】： 龙岩市疾病预防控制中心;福建医科大学龙岩市疾病预防控制中心教学点;福建省疾病预防控制中心;
【基金】：福建省卫生厅青年科研课题(项目号:2012-2-126),题目:病毒性脑炎与肠道病毒感染关系研究
【分类号】：R373
【正文快照】： 埃可病毒(Echovirus,Echo)可引起脑膜炎、脑炎、心肌炎及新生儿严重疾病,于1950年初次分离,属B组肠道病毒(Enterovirus,EV),有许多血清型,Echo 30是肠道病毒性脑炎相关病例常见病原体之一。检测发现龙岩市曾分别发生过由Echo 19型和Echo 6型引起的病毒性脑炎流行[1-2],在2004~，

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