愈癫汤对MK-801致精神分裂症模型大鼠NO信号通路的影响

发布时间：2018-01-31 17:57

本文关键词： 愈癫汤精神分裂症谷氨酸神经元功能障碍 NO信号通路　出处：《中华中医药杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的:基于谷氨酸(Glu)神经元功能障碍理论,探讨愈癫汤对地卓西平(MK-801)致精神分裂症模型大鼠额前脑组织一氧化氮(NO)信号通路的影响。方法:SD雄性成年大鼠随机分为空白组、模型组、对照组及治疗组,每组12只。其中,模型组、对照组及治疗组应用0.1mg/m L的MK-801腹腔注射造模,空白组应用0.9%氯化钠溶液行相同方法腹腔注射,连续14d;于第15天进行干预性给药,对照组给予0.036mg/m L利培酮悬浊液灌胃,治疗组给予浓度0.92g/m L愈癫汤水煎剂灌胃,空白组、模型组给予相应体积蒸馏水灌胃,连续14d。应用分光光度计法、Western blot法及HPLC-ECD检测各组受试大鼠额前脑组织NO活性、一氧化氮合酶(n NOS)蛋白比值及Glu的表达情况。结果:治疗组受试大鼠额前脑组织NO活性、n NOS比值明显低于模型组(P0.05),Glu表达明显高于模型组(P0.05)。结论:愈癫汤可以通过抑制NO活性,降低n NOS比值,稳定Glu在额前脑组织的表达,从而抑制Glu神经毒性,改善Glu神经元功能,达到治疗精神分裂症的目的。
[Abstract]:Objective: based on the theory of glutamate Glu-neuron dysfunction. To investigate the effect of Yuxiangtang on the signal pathway of nitric oxide (no) in frontal forebrain tissue of schizophrenic rats induced by Dizoxipin (MK-801). Methods male adult rats were randomly divided into blank group. Model group, control group and treatment group, 12 rats in each group, model group, control group and treatment group were intraperitoneally injected with 0.1 mg / mL MK-801. The blank group was injected intraperitoneally with 0.9% sodium chloride solution for 14 days. On the 15th day, the control group was given 0.036 mg / mL risperidone suspension, and the treatment group was given 0.92 g / mL Yuxiangshui decoction, blank group. The model group was fed with distilled water of corresponding volume for 14 days. No activity in frontal forebrain of rats in each group was detected by spectrophotometer Western blot and HPLC-ECD. Results: the ratio of nitric oxide synthase (NOS) protein and the expression of Glu were significantly lower in the treatment group than in the model group (P 0.05). The expression of Glu was significantly higher than that of model group P0.050.Conclusion: Yuxiangtang can stabilize the expression of Glu in frontal forebrain by inhibiting the activity of no and decreasing the ratio of n NOS. Thus, the neurotoxicity of Glu was inhibited, the function of Glu neurons was improved, and the treatment of schizophrenia was achieved.
【作者单位】：黑龙江神志医院;黑龙江中医药大学;
【基金】：国家自然科学基金项目(N o.81373776,N o.81473444) 中国博士后科学基金项目(No.2016M601467) 黑龙江省应用技术研究与开发计划项目(No.GZ16C001) 黑龙江省博士后资助项目(No.LBH-Z16180) 黑龙江省中医药中青年科技攻关项目(No.ZQG-075) 黑龙江中医药大学优秀创新人才支持计划项目(2012)~~
【分类号】：R285.5;R-332
【正文快照】： 现代医学认为精神分裂症的发病与谷氨酸(Glu)神经元功能障碍密切相关,大量研究[1-3]均显示,中医药对纠正Glu神经元功能障碍有着特殊的疗效。中医神志病学理论认为,精神分裂症的病位在脑,核心病机为痰瘀互结,脑神失调,笔者据此化裁愈癫汤,在临证中获得了较好的疗效。本研究以Gl

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