植入早期小鼠子宫内膜形态学及Galectin-3、整合素α4表达的研究

发布时间：2018-02-01 10:32

本文关键词： 形态学 Galectin-3 整合素α4 子宫内膜胚胎植入小鼠　出处：《安徽医科大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的①观察动情期非妊娠及植入早期小鼠子宫内膜的光镜结构变化特点;②观察植入早期小鼠子宫内膜Galectin-3和整合素α4的表达分布状况;③探讨Galectin-3和整合素α4在植入早期小鼠子宫内膜表达的关系。方法以成年未经产雌性昆明小鼠为研究对象,雌、雄小鼠按3:1合笼过夜,第二天清晨检查雌鼠阴栓,查到阴栓者记为妊娠第1d。将50只孕鼠随机分为5组,每组10只,分别于妊娠1、2、3、4、5d将5组孕鼠颈椎脱臼处死,剖腹取其子宫,另取10只动情期非妊娠小鼠子宫作对照,4%多聚甲醛固定,分别进行以下处理:①HE染色观察小鼠子宫内膜的光镜结构变化;②免疫组化SP法检测子宫内膜Galectin-3和整合素α4的表达情况;③显微成像系统拍摄图像并进行计算机图像分析系统分析HE染色和免疫组化染色结果。结果①HE染色结果:动情期非妊娠小鼠子宫内膜由腔上皮和固有层组成,固有层中包括子宫腺、基质细胞和血管等,子宫腺为单管状腺,子宫腔上皮和腺上皮均为单层柱状上皮。妊娠1～5d小鼠子宫内膜呈动态变化趋势,主要表现为内膜增厚、增生和水肿,妊娠3～5d固有层中子宫腺体数量渐增多,基质细胞肥大、间隙增大,血管增生。②免疫组化染色结果显示:动情期非妊娠及妊娠1～5d小鼠子宫内膜腺上皮、腔上皮以及基质细胞中均有Galectin-3的表达。妊娠1～5d,Galectin-3在妊娠1～2d小鼠子宫内膜表达逐渐增强,主要表达于子宫内膜腔上皮和腺上皮,妊娠3d起,表达明显增强,妊娠4 d、5d呈强表达,妊娠3～5d Galectin-3在基质细胞中有阳性表达,以妊娠4 d、5d表达最为明显;动情期非妊娠及妊娠1～5d小鼠子宫内膜腺上皮、腔上皮以及基质细胞中均有整合素α4的表达。妊娠1～5d,整合素α4在妊娠1～3d小鼠子宫内膜呈弱阳性表达,主要表达于子宫内膜腔上皮和腺上皮,基质细胞中几乎不表达。妊娠4～5d呈强表达,主要表达于子宫内膜腺上皮、腔上皮和基质细胞胞质中。③计算机图像分析系统计算Galectin-3和整合素α4在妊娠1～5d小鼠子宫内膜表达的平均光密度(MOD)值,经SPSS软件分析,两者在妊娠1～5d小鼠子宫内膜的表达具有正相关性。结论①妊娠1～5d小鼠子宫内膜形态学改变呈动态变化趋势,以“植入窗”期变化最为明显;②妊娠1～5d小鼠子宫内膜腔上皮、腺上皮和基质细胞的胞质中持续表达Galectin-3,以妊娠4～5d表达最强,提示Galectin-3可能在小鼠胚胎植入过程中发挥重要作用;③妊娠1～5d小鼠子宫内膜腔上皮、腺上皮和基质细胞的胞质中持续表达整合素α4,以妊娠4～5d表达最强,提示整合素α4可能参与了小鼠胚胎植入过程;④Galectin-3和整合素α4在妊娠1～5d小鼠子宫内膜的表达具有正相关性,两者可能协同参与了胚胎植入过程。
[Abstract]:Objective 1 to observe the changes of light microscopic structure of endometrium in non-pregnant and early implantation mice during estrous period; 2the expression and distribution of Galectin-3 and integrin 伪 4 in mouse endometrium at the early stage of implantation were observed. 3 to investigate the relationship between Galectin-3 and integrin 伪 4 expression in early implantation of mouse endometrium. Methods female Kunming mice were studied. Female and male mice were treated with 3: 1 cage for the night. The female mice were examined for Yin suppository the next morning. 50 pregnant rats were randomly divided into 5 groups (10 rats in each group). The cervical vertebrae dislocated and the uterus were taken by laparotomy on the 5th day of pregnancy. The uterus of 10 non-pregnant mice in estrous period was fixed with 4% paraformaldehyde. The changes of light microscopic structure of mouse endometrium were observed by the following treatment: 1 he staining. 2Immunohistochemical SP method was used to detect the expression of Galectin-3 and integrin 伪 4 in endometrium. (3) the microscopic imaging system was used to take the images and the computer image analysis system was used to analyze the results of HE staining and immunohistochemical staining. Results 1HE staining showed that the endometrium of non-pregnant mice in estrus was composed of luminal epithelium and lamina propria. The lamina propria included uterine gland, stromal cells and blood vessels, and the uterine gland was single tubular gland. The uterine cavity epithelium and glandular epithelium were monolayer columnar epithelium. On the 3rd and 5th day of pregnancy, the number of uterine glands in lamina propria gradually increased, the stromal cells were hypertrophy and the space was enlarged. The results of immunohistochemical staining showed that the endometrium glandular epithelium of non-pregnant mice during estrous period and that of mice on the 1st and 5th day of gestation. The expression of Galectin-3 was observed in the luminal epithelium and stromal cells, and the expression of Galectin-3 was gradually increased on the 1st and 5th day of gestation in the endometrium of mice on the 1st and 2nd day of gestation. Mainly expressed in endometrial cavity epithelium and glandular epithelium, the expression increased obviously from the 3rd day of gestation, and was strongly expressed on the 4th day of gestation. The positive expression of Galectin-3 was found in stromal cells on the 3rd and 5th day of gestation, especially on the 4th day of gestation. Integrin 伪 4 was expressed in endometrial glandular epithelium, luminal epithelium and stromal cells of non-pregnant and 1-day pregnant mice during estrus. The expression of integrin 伪 4 was weakly positive in the endometrium of mice on the 1st and 3rd day of gestation, mainly in the endometrial cavity epithelium and glandular epithelium, but hardly expressed in stromal cells. Mainly expressed in endometrial glandular epithelium. The mean optical density of Galectin-3 and integrin 伪 4 expression in the endometrium of mice on the 1st day of gestation was calculated by computer image analysis system in the cytoplasm of the lumen epithelium and stromal cells. The results of SPSS software showed that there was a positive correlation between the expression of them in the endometrium of mice on the 1st day of gestation. Conclusion (1) the morphological changes of endometrium in mice on the 1st day of gestation showed a dynamic trend, especially in the period of "implantation window". 2Galectin-3 was continuously expressed in the cytoplasm of endometrial cavity epithelium, glandular epithelium and stromal cells of mice on the 1st day of gestation, and the highest expression was observed on the 5th day of gestation. The results suggest that Galectin-3 may play an important role in mouse embryo implantation. 3 integrin 伪 4 was continuously expressed in the cytoplasm of endometrial cavity epithelium, glandular epithelium and stromal cells of mice on the 1st and 5th day of gestation, and the highest expression was observed on the 5th day of gestation. These results suggest that integrin 伪 4 may be involved in the process of mouse embryo implantation. 4the expression of Galectin-3 and integrin 伪 4 was positively correlated with the expression of integrin 伪 4 in the endometrium of mice on the 1st day of gestation, which may be involved in the process of embryo implantation.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R321

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