脂肪源间充质干细胞移植对D-半乳糖衰老模型大鼠肾、肺组织中衰老相关蛋白表达的影响

发布时间：2018-02-03 15:13

本文关键词： 间充质干细胞移植衰老模型衰老相关基因大鼠　出处：《郑州大学学报(医学版)》2016年03期 　论文类型：期刊论文

【摘要】：目的:探讨脂肪源间充质干细胞(ADMSCs)静脉移植对D-半乳糖衰老模型大鼠的抗衰老效果。方法:18只SD大鼠随机平均分为3组,模型组与移植组大鼠连续8周腹腔注射D-半乳糖[100 mg/(kg·d)],对照组腹腔注射生理盐水。然后移植组尾静脉注射ADMSCs(4×106个),模型组和对照组尾静脉注射生理盐水。4周后,采用Morris水迷宫实验检测大鼠空间学习记忆能力,并测定大鼠血清中超氧化物歧化酶(SOD)活性,并用Western blot法检测大鼠肾与肺组织中衰老相关蛋白沉默信息调节因子1(Sitr1)、P16、P21及增殖细胞核抗原(PCNA)的表达。结果:与对照组相比,模型组大鼠空间学习记忆能力降低(P0.05);与模型组相比,移植组大鼠空间学习记忆能力明显改善(P0.05)。与对照组相比,模型组大鼠血清中SOD活性降低,肾与肺组织中Sirt1、PCNA蛋白表达水平降低(P0.05),P21、P16蛋白表达水平升高(P0.05);与模型组相比,移植组大鼠血清中SOD活性增加(P0.05),肾与肺组织中Sirt1、PCNA蛋白表达水平升高(P0.05),P21、P16蛋白表达水平降低(P0.05)。结论:ADMSCs静脉移植治疗抗衰老作用明显,其可能通过调节衰老相关蛋白表达发挥作用。
[Abstract]:Objective: to investigate the anti-aging effect of adipose derived mesenchymal stem cells (ADMSCs) vein transplantation on aging rats with D-galactose. Methods: eighteen SD rats were randomly divided into 3 groups. Rats in model group and transplantation group were injected intraperitoneally with D-galactose for 8 weeks. [(100 mg/(kg 路d), the control group was intraperitoneally injected with normal saline, and then the transplantation group was injected with ADMSCs(4 脳 106 via caudal vein. Morris water maze test was used to detect the spatial learning and memory ability and the activity of superoxide dismutase (SOD) in serum of the model group and the control group. Western blot method was used to detect the expression of senescence related protein silencing signal regulatory factor (Sitr1) and P16 in rat kidney and lung tissues. Results: compared with the control group, the spatial learning and memory ability of the model group was lower than that of the control group. Compared with the model group, the ability of spatial learning and memory in the transplantation group was significantly improved (P 0.05). Compared with the control group, the activity of SOD in serum of the model group decreased, and the Sirt1 in the kidney and lung tissues. The expression level of PCNA protein decreased in P0.05, P0.01, P16, and increased in P0.05. Compared with the model group, the serum SOD activity in the transplantation group was increased (P 0.05), and the expression of Sirt1 protein in the kidney and lung tissues was increased. Conclusion intravenous transplantation of #en0# ADMSCs has a significant effect on anti-aging, which may play a role by regulating the expression of senescence related protein.
【作者单位】：郑州大学附属郑州中心医院转化医学中心;郑州大学生命科学学院;郑州大学附属郑州中心医院检验科;郑州大学基础医学院药理学教研室;
【基金】：国家自然科学基金资助项目81471306
【分类号】：R-332;R592
【正文快照】： [12]MELINO M,GADD VL,WALKER GV,et al.Macrophagesecretory products induce an inflammatory phenotype inhepatocytes[J].World J Gastroenterol,2012,18(15):1732[13]TITOS E,CL妾RIA J,PLANAGUM妾A,et al.Inhibition of5-lipoxygenase induces cell growth arrest and a

【相似文献】