小鼠流感病毒性肺炎模型中肠道的病变及在中医理论研究中的应用

发布时间：2018-02-08 15:19

本文关键词： 流感病毒肺炎肠道免疫病理肺与大肠相表里　出处：《世界中医药》2016年02期 　论文类型：期刊论文

【摘要】：目的:建立小鼠流感性肺炎模型,明确呼吸道流感病毒感染过程中是否存在特异性的肠道病理损伤,并探讨了其在"肺与大肠相表里"研究中的应用。方法:BALB/c小鼠48只随机分为正常组、病毒组。病毒组给予50μL、50LD50病毒液滴鼻建立流感病毒感染的小鼠肺炎模型,感染后第3、5、6天分别取材,HE染色检测肺脏、大肠、小肠、心脏、肝脏、肾脏组织病理结果,血生化检测血清谷草转氨酶(AST)、谷丙转氨酶(ALT)和尿素氮(BUN)水平。结果:流感病毒感染后,病毒组小鼠肺组织出现明显的炎性病变外,随时间延长肺组织充血水肿出血加重;小肠黏膜腺体排列紊乱,绒毛粗大、部分脱落,黏膜固有层炎性细胞浸润;大肠黏膜固有层、肌层大量炎性细胞浸润,腺体结构消失、脱落;而心脏、肝脏、肾脏无明显病理改变;病毒组小鼠血清AST、ALT、BUN较正常组相比,差异无统计学意义。结论:小鼠流感病毒性肺炎模型中存在小肠、大肠特异性免疫病理损伤,是研究中医"肺与大肠相表里"理论及相关临床治法的理想模型。
[Abstract]:Objective: to establish a mouse model of influenza pneumonia and determine whether there is specific intestinal pathological injury in the process of respiratory influenza virus infection. In this paper, we also discussed its application in the study of "lung and large intestine". Methods Forty-eight BALB / c mice were randomly divided into normal group and virus group. The viral group was given 50 渭 L of 50LD50 virus solution to establish the pneumonia model of influenza virus infection in mice. On the 3rd 5th day after infection, HE staining was used to detect the pathological results of lung, large intestine, small intestine, heart, liver and kidney, and the serum levels of aspartate, alanine aminotransferase (alt) and urea nitrogen bun (bun) were detected in blood biochemistry. Results: after infection of influenza virus, serum alanine aminotransferase (alt) and urea nitrogen bun (bun) were detected. In the viral group, there were obvious inflammatory lesions in the lung tissue, and the hyperemia and edema and hemorrhage of the lung tissue became more severe with the prolongation of time, the intestinal mucosal glands arranged in disorder, the villi were coarse, some of them fell off, the inflammatory cells of the lamina propria were infiltrated, and the lamina propria of the large intestine, A large number of inflammatory cells infiltrated in the myometrium, the structure of the glands disappeared and shed off, while the heart, liver and kidney had no obvious pathological changes. The serum ASTT alt bun in the virus group was higher than that in the normal group. Conclusion: there are small intestine and large intestine specific immunopathologic damage in mouse model of influenza virus pneumonia, which is an ideal model for studying the theory of "lung and large intestine" and related clinical treatment methods in traditional Chinese medicine.
【作者单位】：北京中医药大学基础医学院;
【基金】：国家自然科学基金面上项目(编号:81473520)
【分类号】：R285.5;R-332

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