采用植入式生理信号无线遥测系统记录清醒大鼠自由活动状态下胃的肌电信号

发布时间：2018-02-08 16:50

  本文关键词： 植入式生理信号无线遥测系统 胃肌电 慢波和快波 多潘立酮　出处：《首都医科大学学报》2017年02期 　论文类型：期刊论文

【摘要】：目的为了减少麻醉对胃肌电活动的影响,优化在体胃肌电活动的记录方法,更好地反映自然状态下胃肌电活动状况,减小手术操作对动物的创伤,以及提高术后动物存活率。方法通过植入式生理信号无线遥测系统,结合优化记录电极植入方式将植入子电极固定在胃窦浆膜层,术后动物恢复7 d后,进行胃肌电信号采集,分别采集了清醒、自由活动状态下大鼠胃的肌电活动信号,并对其消化期与消化间期胃肌电活动的频率、幅度及特点进行了分析。结果在消化期,可采集到持续、稳定的胃肌电信号,大鼠胃慢波幅度为(14.39±1.12)μV,频率为(4.76±0.09)次/min,快波幅度为(70.76±5.31)μV,每个慢波上的快波数为10.38±1.22;在消化间期,可观测到周期性变化的移行性复合肌电波形,每个周期持续时间为10~12 min,其Ⅲ相胃肌电活动的慢波幅度为(14.75±0.76)μV,频率为(4.90±0.14)次/min,快波幅度为(72.58±3.67)μV,每个慢波上的快波数为12.00±1.35。在消化期,采用促动力药多潘立酮可以增强胃慢波和快波的幅度及快波的频率,其慢波幅度从(16.87±1.05)μV变为(25.77±2.16)μV(P0.01,n=6),频率从(4.97±0.18)次/min变为(4.85±0.14)次/min(P0.05,n=6),快波幅度从(72.25±3.65)μV变为(104.00±7.72)μV(P0.01,n=6),每个慢波上的快波个数从10.50±1.12变为14.00±0.97(P0.05,n=6)。结论通过植入式生理信号无线遥测系统可以较好地采集到清醒自由活动状态下大鼠胃的慢波及快波电信号,为研究生理及病理生理状态下胃肌电活动提供了一种新手段。
[Abstract]:Objective in order to reduce the influence of anesthesia on gastric myoelectric activity, to optimize the recording method of gastric myoelectric activity in vivo, to better reflect the state of gastric myoelectric activity in natural state, and to reduce the trauma caused by operation. Methods the implanted sub-electrodes were fixed in the antral serosal layer by means of implanted physiological signal wireless telemetry system and optimized recording electrode implantation. After 7 days of recovery, the gastric myoelectric signals were collected. The signals of gastric myoelectric activity in conscious and free activity rats were collected, and the frequency, amplitude and characteristics of gastric myoelectric activity during digestion and interdigestive period were analyzed. The amplitude of gastric slow wave was 14.39 卤1.12 渭 V, the frequency was 4.76 卤0.09 times / min, the amplitude of fast wave was 70.76 卤5.31 渭 V, the number of fast wave on each slow wave was 10.38 卤1.22. The duration of each cycle was 10 ~ 12 min. The amplitude of slow wave of phase 鈪，

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