高脂血症痰证动物模型实验研究及思考

发布时间：2018-02-10 07:52

本文关键词： 高脂喂养高脂血症痰证动物模型模型评价　出处：《中华中医药学刊》2017年09期 　论文类型：期刊论文

【摘要】：目的:采用高脂饲料喂养建立SD大鼠高脂血症痰证动物模型。方法:第1次实验将30只8周龄SD雄性大鼠随机分为正常组10只及模型组20只,分别给予正常饲料和高脂饲料喂养,自由饮食及饮水,于第4、6、8周末,检测血清胆固醇(TC)、总甘油三酯(TG)、低密度脂蛋白(LDL-C)及高密度脂蛋白(HDL-C)水平,8周末采血后处死动物并取出完整肝脏,称量湿重后做HE染色病理切片。第2次实验直接将8只正常组和8只模型组大鼠分别喂养8周,8周末检测大鼠的血脂情况,并将两次实验8周末血脂水平进行比较。实验过程中对大鼠的一般情况进行观察并记录。结果:第1次实验中模型组大鼠与正常组相比,第4、6周末TC升高,差异有统计学意义(P0.05),其余3项无统计学差异,8周末血脂四项与正常组相比均无差异,模型组肝脏病理切片有明显脂肪样变,两组主动脉切片均无明显异常;第2次实验第8周末,模型组大鼠TC、TG升高,HDL-C下降,差异有统计学意义(P0.05),LDL-C无差异。两次实验的结果具有显著差异(P0.05)。两次实验模型组均出现了体胖、倦怠、饮食饮水减少的情况,初步判断符合痰证的证候特点。结论:采用高脂饲料喂养SD大鼠能初步建立高脂血症痰证动物模型,但其造模时间的长短及痰证证候的判断还有待进一步探索和完善,所造动物模型的稳定性和可重复性需要进一步探究。
[Abstract]:Objective: to establish an animal model of hyperlipidemia and phlegm syndrome in SD rats fed with high fat diet. Methods: thirty 8-week-old SD male rats were randomly divided into normal group (n = 10) and model group (n = 20). Normal diet and high fat feed, free diet and drinking water, respectively, for the 8th week of the 4th week, The levels of serum cholesterol, total triglyceride, low density lipoprotein (LDL-C) and high density lipoprotein (HDL-C) were measured. After 8 weeks of blood collection, the animals were killed and the whole liver was taken out. In the second experiment, 8 normal rats and 8 model rats were fed for 8 weeks and 8 weeks, respectively. The blood lipid levels were compared at the end of the 8th week of the two experiments. The general conditions of the rats were observed and recorded during the experiment. Results: in the first experiment, TC in the model group was higher than that in the normal group at the end of week 4 and 6. The difference was statistically significant (P 0.05), and there was no significant difference in the other three items. At the end of 8 weeks, there were no significant differences among the four items of blood lipids compared with the normal group. There were obvious fatty changes in the pathological sections of the liver in the model group and no obvious abnormality in the aortic sections of the two groups. At the end of the 8th week of the second experiment, the TG in the model group increased and HDL-C decreased, but there was no significant difference in LDL-C between the two groups. There was significant difference in the results of the two experiments (P 0.05). Conclusion: hyperlipidemia animal model of phlegm syndrome can be established by feeding SD rats with high fat diet, but the duration of the model and the judgement of phlegm syndrome syndrome need to be further explored and improved. The stability and repeatability of the animal models need to be further explored.
【作者单位】：广州中医药大学基础医学院;
【基金】：国家重点基础研究发展计划(973计划)项目(2014CB542901)
【分类号】：R259;R-332

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