芍药内酯苷及芍药苷对慢性束缚应激配合孤养肝郁模型大鼠行为学及下丘脑-垂体-肾上腺轴的影响

发布时间：2018-02-12 05:56

本文关键词： 芍药内酯苷芍药苷慢性束缚应激行为学下丘脑-垂体-肾上腺轴　出处：《中华中医药杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的:观察芍药内酯苷(AF)、芍药苷(PF)对慢性束缚应激(CRS)配合孤养肝郁模型大鼠行为及下丘脑-垂体-肾上腺(HPA)轴的影响,探讨白芍柔肝解郁作用机制及物质基础。方法:将SD雄性大鼠,根据蔗糖水消耗量及体质量随机分组,每组12只。除空白组外,其余均采用CRS配合孤养复制肝郁模型。观察体质量,行为学指标,并用放射免疫法(RIA)检测大鼠下丘脑促肾上腺皮质激素释放素(CRH)、血浆促肾上腺皮质激素(ACTH)、皮质酮(CORT)的含量。结果:造模第14天,与模型组比较,AF 30mg/kg组、PF 30mg/kg组大鼠体质量明显增加(P0.05,P0.01)。造模第21天,与模型组比较,AF 30mg/kg组、PF 30mg/kg组大鼠体质量,旷场实验中穿行次数明显增加(P0.05,P0.01)而滞留时间显著减少(P0.01),高架十字迷宫实验OE和OT显著增加(P0.05,P0.01),而CT显著减少(P0.01)。AF 30mg/kg组、PF 30mg/kg组大鼠下丘脑CRH、血浆ACTH、CORT的含量均显著降低(P0.05)。结论:AF和PF作为白芍的主要有效成分,不仅可在一定程度上改善了CRS肝郁模型大鼠的行为表现,而且使相关应激激素的含量变化呈现不同的趋势,提示二者均为白芍柔肝解郁作用的有效成分,其作用机制与抑制CRS肝郁模型大鼠HPA轴的激活有关。
[Abstract]:Objective: to observe the effects of Paeoniflorin (AFG) and Paeoniflorin (PFF) on the behavior of chronic restraint stress (CRS) and the hypothalamus-pituitary-adrenal (HPA) axis in rats with solitary liver depression. Methods: SD male rats were randomly divided into 12 rats in each group according to sucrose water consumption and body mass. The rest were treated with CRS combined with solitary nourishment to make liver depression model. Body mass and behavioral indexes were observed. The contents of corticotropin releasing hormone (CRH), plasma adrenocorticotropic hormone (ACTH) and corticosterone (Cort) in the hypothalamus of rats were determined by radioimmunoassay (RIA). Compared with the model group, the body weight of rats in the AF 30 mg / kg group increased significantly in PF 30 mg / kg group, and the body weight of the PF 30 mg / kg group was significantly increased on the 21st day of model making and the PF 30 mg / kg group compared with the model group. In open field experiment, the number of cross passage was significantly increased (P 0.05 mg / kg), and the retention time was significantly decreased. In elevated cross labyrinth test, OE and OT increased significantly (P 0.05 P 0.01), while CT significantly decreased the content of hypothalamus CRH and plasma ACTHCORT in PF 30 mg / kg group (P 0.01 路AF 30 mg / kg). Conclusion the main effective components of Radix Paeoniae Alba are: AF and PF. It can not only improve the behavior of rats with CRS liver depression to a certain extent, but also make the changes of related stress hormones present a different trend, which suggests that both of them are effective components of the effect of Radix Paeoniae Paeoniae Alba on relieving liver depression. The mechanism is related to the inhibition of activation of HPA axis in CRS liver depression model rats.
【作者单位】：北京中医药大学;
【基金】：国家自然科学基金面上项目(No.81473370)~~
【分类号】：R285.5;R-332

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