10%水合氯醛与2%戊巴比妥钠在建立术后疲劳综合征大鼠模型中的麻醉效果比较

发布时间：2018-02-16 10:49

本文关键词： 术后疲劳综合征大鼠模型水合氯醛戊巴比妥钠　出处：《广东医学》2017年12期 　论文类型：期刊论文

【摘要】：目的比较10%水合氯醛与2%戊巴比妥钠在建立术后疲劳综合征(POFS)大鼠模型中的麻醉效果。方法采用70%中段小肠切除后端端吻合法建立术后疲劳综合征大鼠模型。将SPF级健康雄性大鼠24只,随机分为10%水合氯醛组(A组)、2%戊巴比妥钠组(B组)麻醉,观察比较麻醉指标(麻醉死亡率、麻醉状态、麻醉时间、首剂麻醉成功率),术后疲劳综合征评测指标(术前、术后6 h、2、4、6、8 d大鼠的肛温,术后第2~7天进行水迷宫实验结果,术后1、3、5、7 d测试最大抓力结果)。结果两组大鼠的死亡率未见明显差异,与B组比较,A组麻醉诱导时间和深度麻醉时间更短(P0.05),术后肛温恢复更快(P0.05),最大抓力在术后第1、3、5、7天明显更大(P0.05),在水迷宫实验中总平均逃避潜伏期明显更短(P0.05),穿越平台次数明显更多(P0.05)。结论在POFS大鼠模型制备中,与2%戊巴比妥相比较,采用10%水合氯醛麻醉,麻醉诱导时间更短,深度麻醉时间适中,首剂麻醉成功率高。麻醉可能对POFS的程度有一定影响,选用10%水合氯醛作为麻醉剂,大鼠模型的POFS程度更轻,质量控制较好。
[Abstract]:Objective to compare the anesthetic effect of 10% chloral hydrate and 2% pentobarbital sodium in the establishment of the rat model of postoperative fatigue syndrome (POFS). 24 healthy SPF male rats, 10% chloral hydrate group was randomly divided into 2% pentobarbital sodium group (group B). The anaesthesia indexes (mortality rate, state of anesthesia, time of anesthesia, success rate of first dose anesthesia, postoperative fatigue syndrome index before operation) were observed and compared. The anus temperature of rats was measured at 6 hours after operation. The results of water maze test were performed on the 2nd day and 7th day after operation. The maximum grasping force was measured at 1: 3 and 5 ~ 5 days postoperatively. Results there was no significant difference in mortality between the two groups, and there was no significant difference in the mortality between the two groups. Compared with group B, the induction time of anesthesia and the time of deep anesthesia in group A were shorter than those in group B, the anus temperature recovered faster after operation and the recovery of anus temperature was faster than that in group B. The maximum grasping power was significantly larger than that in group B on the 7th day after operation. The total average escape latency in water maze experiment was significantly shorter than that in water maze experiment (P 0.05). Conclusion in the preparation of POFS rat model, the frequency of P0.05 is more than that of P0.05.Conclusion in the preparation of POFS rat model, Compared with 2% pentobarbital, 10% chloral hydrate anesthesia had shorter induction time, moderate depth anesthesia time and high success rate of first dose anesthesia. Anesthesia may have some influence on the degree of POFS. 10% chloral hydrate was selected as anesthetic agent. The POFS of rat model was lighter and the quality control was better.
【作者单位】：广州中医药大学第二临床医学院;广东省中医院芳村医院微创外科;
【基金】：广东省科技计划项目(编号:2013B032500010)
【分类号】：R-332;R614

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