甲强龙联合脂多糖诱导兔股骨头坏死模型的建立

发布时间：2018-02-17 06:14

本文关键词： 股骨头坏死甲强龙脂多糖兔　出处：《中国骨质疏松杂志》2016年04期 　论文类型：期刊论文

【摘要】：目的观察甲强龙联合脂多糖诱导兔股骨头坏死的实验效果。方法 30只健康雄性新西兰兔,随机分为实验组(20只)和对照组(10只),实验组耳缘静脉注射脂多糖(lipopolysaccharides,LPS)10μg/kg,24 h后肌注甲强龙20 mg/kg,1次/d,共3 d。对照组注射同等剂量的生理盐水。6周后观察兔股骨头X射线、磁共振及病理学等指标的改变。结果对照组X线:双侧股骨头骨皮质完整,大小及形态正常,骨密度均匀,骨小梁清晰;实验组X线:兔股骨头密度减低,骨小梁模糊不清,可见一囊状透亮区,股骨头保持完整;MRI示:股骨头见一类圆形高信号影,边界相对清晰,关节面完整;病理:骨小梁纤细,部分骨小梁断裂,髓腔内造血细胞减少,脂肪细胞体积增大,有的融合成泡状。结论甲强龙联合脂多糖能够成功安全便捷地诱导兔股骨头坏死动物模型。
[Abstract]:Objective to observe the effect of methylenolone combined with lipopolysaccharide on femoral head necrosis in rabbits. The experimental group (n = 20) and the control group (n = 10) were randomly divided into two groups: the control group (n = 10) and the control group (n = 10) were injected intravenously with lipopolysaccharide polysaccharide and lipopolysaccharide lipopolysaccharide (LPS) 10 渭 g 路kg ~ (-1) L ~ (-1) for 24 h after intramuscular injection of mestrone 20 mg 路kg ~ (-1) per day for 3 days. The control group was injected with the same dose of physiological saline for 6 weeks. Results in the control group, the bone cortex was intact, the size and shape were normal, the bone density was uniform, the trabecular bone was clear in the control group, and the density of the femoral head in the experimental group was decreased, and the trabecular bone was blurred. A clear cystic area with intact femoral head was observed on MRI: a class of circular hyperintense images were seen in femoral head, the boundary was relatively clear, and the articular surface was intact, pathology showed that trabeculae were slender, some trabeculae were broken, and hematopoietic cells in medullary cavity were decreased. Conclusion Methylenolone combined with lipopolysaccharide can successfully and safely induce rabbit model of femoral head necrosis.
【作者单位】：南京中医药大学骨伤研究所骨伤修复与重建新技术实验室;南京中医药大学附属医院骨伤科;
【基金】：江苏省“六大人才高峰”资助项目(2011-WS039D) 江苏省高校优势学科建设工程资助项目江苏省中医药局科技项目(LZ09039)
【分类号】：R681.8;R-332

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