表皮葡萄球菌icaA和icaD基因表达及其与黏质物形成的关系

发布时间：2018-02-22 04:04

本文关键词： 表皮葡萄球菌黏质物 icaA基因 icaD基因　出处：《中国感染与化疗杂志》2012年01期 　论文类型：期刊论文

【摘要】：目的了解临床分离的表皮葡萄球菌所携带的icaA和icaD操纵子表达情况及其与黏质物形成之间的关系。方法收集ICU临床分离的表皮葡萄球菌62株,采用刚果红法检测其黏质物形成,PCR法检测icaA、icaD基因表达,卡方检验法进行统计学处理。结果 62株表皮葡萄球菌中产黏质物菌株有26株(41.9%);产黏质物菌株中icaA、icaD基因表达阳性的菌株分别为22株(84.6%)和19株(73.1%),icaA、icaD基因表达均为阳性有17株(65.4%),明显高于非产黏质物菌株(P0.01);icaA、icaD基因表达均阴性为2株(7.7%),明显低于非产黏质物菌株(P0.01)。结论 icaA、icaD基因表达对表皮葡萄球菌的黏质物产生具有重要影响,但并不是其唯一的影响因素。
[Abstract]:Objective to investigate the expression of icaA and icaD operons carried by Staphylococcus epidermidis and their relationship with mucus formation. Methods 62 strains of Staphylococcus epidermidis isolated from ICU were collected. Congo red assay was used to detect the expression of icaAgnicaD gene by polymerase chain reaction (PCR). Results among the 62 strains of Staphylococcus epidermidis, 26 strains were found to be myxin-producing strains, and 22 strains were positive for the expression of icaAnica D gene, respectively, and 19 strains were positive for the expression of ICA AgnicaD gene in S. epidermidis, and 19 strains of S. epidermidis were found to be positive for the expression of ICA AgnicaD gene in the 62 strains of Staphylococcus epidermidis by chi-square test. There were 17 strains with 65.4B, which were significantly higher than that of non-mucotrophic strain (P0.01). The negative expression of ICA-AoicaD gene was significantly lower than that of non-slime producing strain P0.010.Conclusion the expression of ICA AgnicaD gene has an important effect on the mucin production of Staphylococcus epidermidis, and is significantly lower than that of non-slime producing strain (P0.01), which is significantly lower than that of non-slime producing strain (P0.01). But it is not the only factor affecting it.
【作者单位】：安徽医科大学附属省立医院ICU;安徽医科大学附属省立医院微生物实验室;
【基金】：安徽省教育厅课题基金资助项目(KJ2009B001Z)
【分类号】：R378.11

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