良性前列腺增生合并迟发性性腺功能减退大鼠模型的构建

发布时间：2018-02-22 09:48

本文关键词： 前列腺增生迟发性性腺功能减退症动物模型环磷酰胺丙酸睾酮　出处：《第二军医大学学报》2017年07期 　论文类型：期刊论文

【摘要】：目的探讨良性前列腺增生(BPH)合并迟发性性腺功能减退症(LOH)动物模型的建立方法,并分析其特点。方法选取80只SD大鼠,随机分为对照组、BPH合并LOH组、BPH组、LOH组,每组均为20只。BPH合并LOH组给予环磷酰胺[20 mg/(kg·d)]腹腔内连续注射5d,而后给予丙酸睾酮[50 mg/(kg·d)]腹腔内连续注射28d;BPH组以同等体积生理盐水代替环磷酰胺,LOH组以同等体积橄榄油代替丙酸睾酮,对照组以同等体积生理盐水代替环磷酰胺、同等体积橄榄油代替丙酸睾酮,给药方法同BPH合并LOH组。停药2d后,进行悬尾实验、负重游泳实验及交配实验,并通过断尾法取血以检测血清睾酮含量。完成全部操作后,通过颈椎脱臼法处死全部实验动物,解剖观察前列腺、称量并行组织病理学检查。结果 LOH组、BPH合并LOH组、对照组、BPH组大鼠平均前列腺指数分别为1.58±0.13、2.93±0.19、2.33±0.13、3.23±0.11,血清睾酮含量分别为(4.91±1.06)、(9.52±1.02)、(12.59±0.70)、(19.69±0.56)ng/mL,悬尾挣扎次数分别为(97.40±15.86)、(120.40±14.06)、(223.83±16.51)、(235.29±18.77)次,各组间差异均有统计学意义(P0.05,P0.01);LOH组、BPH合并LOH组、对照组、BPH组大鼠平均负重游泳时间分别为(74.27±9.29)、(167.47±23.35)、(302.33±30.10)、(261.59±35.13)s,嗅阴次数分别为(1.53±0.52)、(3.07±0.88)、(9.17±1.30)、(9.59±1.12)次,除对照组和BPH组差异无统计学意义外,其余各组间差异均有统计学意义(P0.01);LOH组、BPH合并LOH组、对照组、BPH组大鼠平均骑跨次数分别为(0.33±0.49)、(0.47±0.52)、(2.11±0.47)、(2.29±0.47)次,除对照组与BPH组、BPH合并LOH组与LOH组差异无统计学意义外,其余各组间差异均有统计学意义(P0.01)。结论通过腹腔注射环磷酰胺及丙酸睾酮可获得BPH合并LOH大鼠模型。
[Abstract]:Objective to investigate the method of establishing the animal model of benign prostatic hyperplasia (BPH) with delayed hypogonadism and to analyze its characteristics. Methods Eighty SD rats were randomly divided into two groups: the control group (BPH + LOH group) and the control group (LOH group). 20 rats in each group were given cyclophosphamide [20 mg/(kg 路d] intraperitoneal injection for 5 days, and then testosterone propionate [50 mg/(kg 路d] was injected intraperitoneally for 28 days. The same volume of normal saline was used instead of cyclophosphamide loh group. Oil instead of testosterone propionate, The control group was given the same volume of normal saline instead of cyclophosphamide, the same volume of olive oil instead of testosterone propionate, and the same dosage of BPH combined with LOH group. After 2 days, suspension test, weight-bearing swimming test and mating experiment were carried out. After the complete operation, all the experimental animals were killed by cervical dislocations, the prostate was dissected, the prostate was measured and histopathological examination was performed. Results the LOH group was treated with LOH. The average prostatic index of rats in the control group was 1.58 卤0.131.93 卤0.19 卤2.33 卤0.133.23 卤0.11, and the serum testosterone level was 4.91 卤1.06 卤1.06 卤1.06 卤12.59 卤0.70 卤19.69 卤0.56 ng / mL, respectively. The number of tail struggle was 97.40 卤15.86 ng / mL. 40 卤14.063.83 卤16.51D 235.29 卤18.77). There were significant differences between the two groups. The average weight-bearing swimming time of rats in the control group was 74.27 卤9.29, 167.47 卤23.35, 302.33 卤30.10, 261.59 卤35.13 s, and the number of olfactory negative was 1.53 卤0.52, 3.07 卤0.882.17 卤1.309.59 卤1.12, respectively. Except for the control group and the BPH group, the differences were statistically significant. The average spanking times of rats in the control group were 0.33 卤0.49, 0.47 卤0.52, 2.11 卤0.47, 2.29 卤0.47) respectively, except that there was no significant difference between the control group and the BPH group in the combination of LOH group and LOH group. Conclusion BPH combined with LOH rat model can be obtained by intraperitoneal injection of cyclophosphamide and testosterone propionate.
【作者单位】：第二军医大学长海医院泌尿外科;上海市杨浦区市东医院泌尿外科;第二军医大学长海医院病理科;Division
【基金】：第二军医大学长海医院“1255”学科建设计划(CH125520302)~~
【分类号】：R-332;R588.1;R697.3

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