基于CRISPR数据库的病原菌CRISPR结构分析

发布时间：2018-02-22 10:43

本文关键词： 噬菌体质粒病原菌 CRISPR 间隔序列　出处：《扬州大学学报(农业与生命科学版)》2017年02期 　论文类型：期刊论文

【摘要】：成簇的规律间隔短回文重复序列(CRISPR)是大多数细菌和古菌在生存压力下进化出的一套抵抗噬菌体干扰的防御系统。采用生物信息学方法,首先根据VFDB、PHI-base和NMPDR病原菌数据库确定了CRISPR数据库中2 612株细菌中的549株植物、动物及人体病原菌菌株,然后对病原菌菌株和总菌株的CRISPR结构进行比较分析。结果表明:病原菌菌株中含有CRISPR结构的菌株比例为48.5%,略高于总菌株中的比例;但间隔序列的数量在总菌株和病原菌菌株中的差异显著,病原菌菌株间隔序列分布情况均低于细菌的平均水平;病原菌菌株CRISPR结构中病毒来源的间隔序列比质粒来源所占比例大。通过对病原菌CRISPR结构的分析,为进一步理解病原菌的生活和动植物宿主菌的获得性免疫防御系统提供了理论指导。
[Abstract]:Cris PRR, a regular cluster of short palindrome repeats, is a system of defense against phage interference developed by most bacteria and archaea under survival pressure, using bioinformatics, First of all, 549 strains of plant, animal and human pathogens were identified in CRISPR database according to PHI-base and NMPDR pathogenic bacteria database. Then the CRISPR structure of pathogen strain and total strain were compared and analyzed. The results showed that the proportion of pathogen strain with CRISPR structure was 48.5, which was slightly higher than that of total strain. However, the number of spacer sequences was significantly different between the total strains and the pathogenic strains, and the distribution of the spacer sequences of the pathogens was lower than the average level of the bacteria. The spacer sequence of the virus source in the CRISPR structure of the pathogen strain was larger than that of the plasmid source. The CRISPR structure of the pathogen strain was analyzed. It provides theoretical guidance for further understanding the life of pathogens and the acquired immune defense system of plant and animal host bacteria.
【作者单位】：扬州大学环境科学与工程学院;
【基金】：国家重点基础研究发展计划项目(973-2015CB150503)
【分类号】：R37

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