成纤维细胞生长因子修饰的骨髓间充质干细胞移植对颅脑损伤模型大鼠神经功能的改善机制分析

发布时间：2018-02-22 23:07

本文关键词： 大鼠成纤维细胞生长因子骨髓间充质干细胞颅脑损伤　出处：《临床和实验医学杂志》2016年12期 　论文类型：期刊论文

【摘要】：目的探讨成纤维细胞生长因子修饰的骨髓间充质干细胞移植对颅脑损伤改善的机制。方法从大鼠中分离骨髓间充质干细胞,培养、传代,以腺病毒为载体介导成纤维细胞生长因子转染骨髓间充质干细胞。以Feeney's法建立大鼠颅脑损伤模型60只,随机分为对照组、骨髓间充质干细胞组(BMSC组)和成纤维细胞生长因子-骨髓间充质干细胞组(FGF-modified BMSC组)。采用免疫组化法检测Brd U标记的骨髓间充质干细胞在脑组织内的分布,TUNEL法检测脑组织细胞的凋亡情况。结果培养骨髓间充质干细胞,Western-Blot法显示成纤维细胞生长因子成功转染骨髓间充质干细胞。成纤维细胞生长因子-骨髓间充质干细胞组的Brd U标记阳性细胞数显著性高于骨髓间充质干细胞组(P0.05),而凋亡性细胞个数明显减少(P0.05)。移植14 d后,成纤维细胞生长因子-骨髓间充质干细胞大鼠的Longa神经功能评分显著性低于对照组和骨髓间充质干细胞组(P0.05)。结论骨髓间充质干细胞能很好地存在于受损的大鼠脑内,并能够减少受损组织细胞的凋亡,从而改善颅脑损伤大鼠的神经功能损伤。
[Abstract]:Objective to investigate the mechanism of bone marrow mesenchymal stem cell transplantation modified by fibroblast growth factor (FGF) on the improvement of craniocerebral injury. Methods Bone marrow mesenchymal stem cells (BMSCs) were isolated from rats, cultured and subcultured. Bone marrow mesenchymal stem cells (BMSCs) were transfected with adenovirus mediated fibroblast growth factor (FGF). Sixty rat models of craniocerebral injury were established by Feeney's method and were randomly divided into control group. Bone marrow mesenchymal stem cells (BMSCs) and fibroblast growth factor-bone marrow mesenchymal stem cells (FGF-modified BMSC) were used to detect the distribution of bone marrow mesenchymal stem cells (BMSCs) labeled with Brd U in brain tissue by immunohistochemistry and Tunel method. Results Western-Blot method showed that fibroblast growth factor was successfully transfected into bone marrow mesenchymal stem cells. Brd of fibroblast growth factor-bone marrow mesenchymal stem cells group. The number of U labeled positive cells was significantly higher than that of bone marrow mesenchymal stem cells (BMSCs), while the number of apoptotic cells decreased significantly. The Longa neural function score of fibroblast growth factor-bone marrow mesenchymal stem cell rats was significantly lower than that of control group and bone marrow mesenchymal stem cell group (P 0.05). Conclusion Bone marrow mesenchymal stem cells can well exist in the damaged rat brain. It can reduce the apoptosis of injured tissue cells and improve the nerve function injury of rats with craniocerebral injury.
【作者单位】：上海交通大学附属第六人民医院神经外科;
【基金】：上海市科学技术委员会科研计划项目(编号:13411951401)
【分类号】：R651.15;R-332

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