基于中医方证代谢组学技术的六味地黄丸干预脑瘫大鼠模型研究

发布时间：2018-02-23 11:36

本文关键词： 中医方证代谢组学缺氧缺血脑瘫六味地黄丸药效物质基础代谢物中医药中药血清药物化学代谢组学　出处：《世界科学技术-中医药现代化》2016年10期 　论文类型：期刊论文

【摘要】：目的:本研究基于中医方证代谢组学理论和技术平台,研究六味地黄丸(LW)对脑瘫大鼠模型的干预作用。方法:研究采用延迟剖宫产手术方法复制缺氧缺血脑瘫(HICP)大鼠模型,利用经典行为学方法对HICP模型和LW的干预作用进行评价;最后借助中医方证代谢组学技术揭示了HICP模型大鼠尿液的潜在生物标记物与LW干预HICP模型体内显效成分的相关性。结果:研究表明LW通过调节HICP模型大鼠机体的糖类代谢、氨基酸代谢、核苷酸代谢和甾体激素类生物合成四大类代谢途径,进而有效改善HICP模型大鼠的运动能力,并确定LW的入血成分中6-O-甲基梓醇、莫诺苷、丹皮酚原苷、毛蕊花苷、獐牙菜苷脱水的代谢产物为干预HICP大鼠模型的潜在药效物质基础。本证实了中医方证代谢组学技术可以成为阐明中药药效物质基础的有力手段。
[Abstract]:Objective: to study the effect of Liuwei Dihuang Pill (LW) on cerebral palsy rat model based on the theory and technique of TCM syndromes metabolism. Methods: the model of hypoxic-ischemic cerebral palsy was established by delayed cesarean section in rats. The intervention effects of HICP model and LW were evaluated by classical behavioral methods. Finally, the correlation between the potential biomarkers in urine of HICP model rats and the significant components of LW intervention in HICP model was revealed by the metabolic technique of TCM prescription syndrome. Results: the results showed that LW regulated the carbohydrate metabolism of HICP model rats. Amino acid metabolism, nucleotide metabolism and steroid biosynthesis were used to improve the motor ability of HICP model rats, and to determine the contents of 6-O- methylcatalpol, monoside, paeonol and ruffin in the blood of LW model rats. The metabolites of Swertia luciferin dehydration are the potential substance basis of interfering with HICP rat model. It is proved that the metabolomics technique of TCM prescription syndrome can be a powerful means to clarify the substance basis of TCM pharmacodynamics.
【作者单位】：黑龙江中医药大学国家中医药管理局中医方证代谢组学研究中心/国家中医药管理局中药血清药物化学重点研究室/中美中医方证代谢组学技术合作中心;
【基金】：国家自然科学基金委重点项目(81430093):中药体内药效物质基础的系统分析方法学——中医方证代谢组学研究,负责人:王喜军科学技术部“重大新药创制”国家科技重大专项(2015ZX09101043-011):课题名称:中药经典名方整合作用机制关键技术研究,负责人:王喜军国家自然科学基金委面上项目(81473584):基于新生大鼠脑瘫模型的六味地黄丸治疗“五迟”、“五软”证的作用机理及药效物质基础研究,负责人:王萍
【分类号】：R285.5;R-332

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