电脑辐射对小鼠部分器官组织形态学上的影响

发布时间：2018-02-26 22:07

本文关键词： 电脑辐射血红蛋白空肠形态学 Caveolin-1蛋白　出处：《辽宁师范大学》2011年硕士论文　论文类型：学位论文

【摘要】：本研究把40只4周龄昆明种雄性小白鼠(体重:23.0±2.7g/只)适应性饲养一周后,随机分为两组:A组(对照组)、B组(实验组)每组20只。对照组在室内光源下正常饲养,实验组将辐射强度为0.31μT笔记本电脑架于鼠笼上面,辐射时间为每天上午辐射4小时,下午辐射4小时,中午停止辐射1小时,连续辐射28天后,先用眼眶后静脉丛采血法采血1～2mL后,取睾丸、肾、肺、部分空肠、气管等器官,然后用石蜡切片法制作组织切片并进行H-E染色;利用显微数字图像分析处理系统观察组织结构的变化;血样用沙利氏比色法测定血红蛋白含量;用蛋白含量测量的方法测定小鼠睾丸中的Caveolin-1蛋白含量。以这些指标来研究和探讨电脑辐射对小鼠部分器官组织形态学上的影响,从而探究其对人体各器官组织形态学上的影响及细胞损伤过程中Caveolin-1蛋白的表达变化,为进一步研究细胞损伤机理提供理论基础和科学依据。结果显示: 1.实验组的采食量及饮水量明显(P 0.05)小于对照组;实验组的平均体重从辐射2周后开始明显(P 0.05)小于对照组的平均体重;实验组血红蛋白含量(13.7±0.3g/100mL)明显(P 0.05)少于对照组(15.1±0.3g/100mL)血红蛋白含量。 2.实验组空肠的小肠绒毛排列不规则,变疏松,有的变短,甚至出现尖端断裂脱落等现象,黏膜上皮的吸收细胞出现萎缩及坏死,且吸收细胞游离缘上的纹状缘有脱落现象。 3.实验组气管的软骨萎缩,气管腔里有很多异物,粘膜上皮细胞萎缩,甚至伴有脱落的现象。 4.实验组的细支气管,终末细支气管及呼吸性细支气管的上皮细胞发生萎缩现象,排列变疏松,柱状细胞上缘的纤毛有脱落。部分肺泡管、肺泡囊、肺泡伴有充血,肺泡隔里的纤维明显增生,伴有炎性细胞的浸润。 5.实验组的生精小管中各级生精细胞发生萎缩,数量减少,管腔内精子数量减少且很多精子出现畸形现象。睾丸间质明显减少,间质细胞变得模糊有的甚至消失。 6.实验组肾小球玻璃样变性,纤维化,有些肾小球代偿而肥大,一些肾小管发生萎缩,有些肾小管代偿扩张,间质结缔组织有增生现象。 7.实验组睾丸中Caveolin-1蛋白表达减少。
[Abstract]:In this study, 40 4-week-old Kunming male mice (weight of 23.0 卤2.7 g / mouse) were fed adaptively for one week, and were randomly divided into two groups: group A (control group, n = 20), group B (control group, n = 20) and control group (n = 20). In the experimental group, the radiation intensity of 0.31 渭 T notebook computer was placed on the cage. The radiation time was 4 hours in the morning, 4 hours in the afternoon, 1 hour at noon, and 28 days after continuous radiation. The testis, kidneys, lungs, part of jejunum, trachea and other organs were collected from the blood collected from the posterior orbital venous plexus at 1: 2 mL, and then the tissue sections were made by paraffin section and stained with H-E. The changes of tissue structure were observed by means of microscopic digital image analysis and processing system, and hemoglobin content was determined by hematocrit colorimetry in blood samples. The content of Caveolin-1 protein in testis of mice was measured by protein content measurement. The effects of computer radiation on the morphology of some organs of mice were studied and discussed by using these indexes. In order to provide theoretical basis and scientific basis for further study on the mechanism of cell injury, the effects of Caveolin-1 on the morphology of human organs and tissues and the expression of Caveolin-1 protein in the process of cell injury were investigated. The results show that:. 1. The intake and water intake of the experimental group were significantly lower than that of the control group (P 0.05), and the average body weight of the experimental group was significantly lower than that of the control group from 2 weeks after radiation. The hemoglobin content in the experimental group was 13.7 卤0.3g / 100mL) significantly lower than that in the control group (15.1 卤0.3g / 100mL). 2. In the experimental group, the villi of the jejunum were arranged irregularly and loosened, some of them became shorter, and even the tip was broken off, the resorption cells of the mucosal epithelium were atrophied and necrotized, and the striate edge of the free edge of the resorption cell was shedded. 3. In the experimental group, there were many foreign bodies in the trachea, atrophy of mucosal epithelium and even exfoliation. 4. The epithelial cells of the bronchioles, terminal bronchioles and respiratory bronchioles of the experimental group were atrophied, arranged loosely, and the cilia of the upper edge of the columnar cells shedded. Part of the alveolar duct, alveolar sac, alveolar vesicle, alveolar accompanied by congestion, The fibers in the alveolar septum were proliferated, accompanied by inflammatory cell infiltration. 5. The spermatogenic cells in the seminiferous tubules of the experimental group were atrophied, the number of spermatozoa in the tubules decreased, and many spermatozoa deformed, the interstitial cells of the testis decreased obviously, and some of the interstitial cells became blurred or even disappeared. 6. Glomerular vitreous degeneration, fibrosis, some glomerular compensatory hypertrophy, atrophy of some renal tubules, compensatory dilatation of some tubules and hyperplasia of interstitial connective tissue in the experimental group. 7. The expression of Caveolin-1 protein in testis of experimental group was decreased.
【学位授予单位】：辽宁师范大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R361.2

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