外源性脑源性神经因子对遗传性聋动物模型耳蜗形态和功能的影响

发布时间：2018-02-27 00:31

本文关键词： 外源性脑源性神经因子耳蜗聋遗传性　出处：《临床耳鼻咽喉头颈外科杂志》2017年17期 　论文类型：期刊论文

【摘要】：目的:研究外源性脑源性神经因子(BDNF)对条件敲除缝隙链接蛋白26(Cx26)小鼠耳蜗形态和功能的影响。方法:选取条件敲除的Cx26小鼠为动物模型,在出生后2d(P0-P2)通过中阶注射给予外源性BDNF。分别观察BDNF在耳蜗中的表达情况及其对神经纤维生长的促进作用,注射后60d,BDNF对corti器及螺旋神经节细胞的保护作用,并检测注射后动物听力的改变情况。结果:(1)外源性BDNF可在实验动物体内良好表达;(2)外源性BDNF对动物耳蜗神经纤维生长有良好的促进作用;(3)外源性BDNF可有效保护corti器的形态;计数耳蜗各回螺旋神经节细胞数,以未注射耳顶回的密度值作为标准单位,注射耳中回和底回的密度值分别为0.81±0.02、0.65±0.02,与未注射耳相比,均差异有统计学意义(均P0.05);(4)通过计算注射耳与未注射耳各频率ABR阈值,显示两者差异无统计学意义。结论:早期给予外源性BDNF可有效保护条件敲除Cx26小鼠耳蜗的corti器及螺旋神经节细胞的形态,但对于听功能则无保护作用。
[Abstract]:Objective: to study the effect of exogenous brain-derived neurofactor (BDNF) on cochlea morphology and function in mice with conditional knockout gap link protein 26Cx26. methods: Cx26 mice with conditional knockout were selected as animal models. The expression of BDNF in cochlea and its promoting effect on nerve fiber growth were observed, and the protective effects of BDNF on corti organ and spiral ganglion cells were observed 60 days after injection. Results the exogenous BDNF could well express the growth of cochlear nerve fibers in experimental animals. The exogenous BDNF could promote the growth of cochlear nerve fibers in animals. [results] exogenous BDNF could effectively protect the morphology of the corti organ. The number of spiral ganglion cells in each gyrus of the cochlea was counted. The density of the parietal gyrus was taken as the standard unit. The density values of the middle and bottom gyrus of the injected ears were 0.81 卤0.02 and 0.65 卤0.02, respectively, compared with those of the uninjected ears. The difference was statistically significant (P 0.05). The frequency ABR thresholds of injected and uninjected ears were calculated. Conclusion: early administration of exogenous BDNF can effectively protect cochlear corti organ and spiral ganglion cells of Cx26 mice, but have no protective effect on auditory function.
【作者单位】：南方医科大学南方医院耳鼻咽喉-头颈外科;美国埃默里大学医学院耳鼻咽喉科学系;
【基金】：美国NIH基金(No:NIH ROI-DC006483) 广东省自然科学基金(No:2015A030313303)
【分类号】：R-332;R764.43

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