AQP-1、AQP-4、VEGF、sE-选择素在肺低氧代谢中的表达
本文关键词： 低氧代谢 水通道蛋白-1 水通道蛋白-4 低氧代谢 血管内皮生长因子 可溶性E选择素 出处：《广西医科大学》2012年硕士论文 论文类型：学位论文
【摘要】：目的：研究肺低氧代谢中大鼠肺组织水通道蛋白1、4(AQP-1、4)的表达,探讨低氧代谢与其相关性。 方法：将30只SD大鼠随机分成对照组和实验组A、B、C、D组,即1/2h单肺低氧组、1h单肺低氧组、2h单肺低氧组、4h单肺低氧组。对照组采用气管切开插管行双肺通气,实验组采用气管切开插管行单肺通气,建立急性低氧性肺损伤模型。分别于通气1/2h、1h、2h、4h后取非通气侧肺组织,光镜下观察肺组织病理变化情况；免疫组化观察AQP-1、AQP-4蛋白表达与分布；采用实时荧光定量PCR测定大鼠AQP-1、AQP-4mRNA表达。 结果：光镜下可见实验组随着低氧时间延长,肺组织毛细血管充血,腔内充满较多红细胞,肺泡隔增宽,炎性细胞浸润,而对照组未见明显病理变化。肺组织免疫组化观察到随着低氧时间延长AQP-1表达明显减少,而AQP-4变化不大。单肺低氧4h组AQP-lmRNA的表达较对照组显著减少(P0.05),且随着低氧时间延长呈下降趋势：ABCD,而AQP-4的表达没有明显变化。 结论：长时间低氧代谢导致大鼠肺组织结构发生改变,AQP-1表达下调,且与低氧代谢时间呈负相关,而AQP-4与肺低氧代谢无相关性。 目的：研究肺低氧代谢中大鼠血管内皮生长因子(VEGF)、可溶性E选择素(sE-Selectin)的表达,探讨低氧代谢与其相关性。 方法：将30只SD大鼠随机分成对照组和实验组A、B、C、D组,即1/2h单肺低氧组、1h单肺低氧组、2h单肺低氧组、4h单肺低氧组。对照组采用气管切开插管行双肺通气,实验组采用气管切开插管行单肺通气,建立急性低氧性肺损伤模型。分别于通气1/2h、1h、2h、4h后取非通气侧大鼠肺组织及血清。光镜下观察肺组织病理变化情况,采用ELISA法测定血清中sE-选择素、肺组织和血清中VEGF的含量,免疫组化观察VEGF蛋白表达与分布,采用实时荧光定量PCR测定大鼠VEGFmRNA表达。 结果：HE观察到实验组随着低氧时间延长,肺组织毛细血管充血,腔内充满较多红细胞,肺泡隔增宽,炎性细胞浸润,而对照组未见明显病理变化。ELISA法观察到sE-选择素在大鼠单肺低氧1h、2h、4h血清中的表达较对照组显著升高(P0.05),且随着时间延长,含量增加；VEGF在大鼠单肺低氧4h组肺组织中的表达较正常组显著降低(P0.05),而在该组血清中的表达较对照组显著升高(P0.05)。肺组织免疫组化观察到随着低氧时间延长VEGF表达减少。单肺低氧4h组VEGF mRNA的表达较对照组显著减少(P0.05),且随着低氧时间延长呈下降趋势：ABCD。 结论：长时间低氧代谢导致大鼠肺组织结构发生改变,sE-选择素表达上调,且与低氧代谢时间呈正相关；而肺组织中VEGF与低氧代谢时间呈负相关。
[Abstract]:Aim: to study the expression of aquaporin 1 (aquaporin 1) in rat lung tissue during hypoxic metabolism, and to explore the correlation between hypoxia metabolism and AQP-4. Methods: thirty Sprague-Dawley rats were randomly divided into two groups: control group and experimental group, namely 1 / 2 h one-lung hypoxia group, 1 / 2 h single lung hypoxia group, 1 h single lung hypoxia group and 4 h single lung hypoxia group, the control group was treated with tracheotomy and intubation to perform double lung ventilation. The model of acute hypoxic lung injury was established by using tracheotomy and intubation to establish acute hypoxic lung injury model. The lung tissues of the non-ventilated side were taken for 4 h after 1 / 2 h of ventilation, and the pathological changes of lung tissue were observed under light microscope, and the expression and distribution of AQP-1AQP-4 protein were observed by immunohistochemistry. The expression of AQP-4 mRNA in rat AQP-1 was measured by real-time fluorescence quantitative PCR. Results: with the prolongation of hypoxic time, pulmonary capillary congestion, more red blood cells, wider alveolar septum and inflammatory cell infiltration were observed under light microscope in the experimental group. In the control group, there was no obvious pathological change. The expression of AQP-1 decreased with the prolongation of hypoxic time by immunohistochemistry. Compared with the control group, the expression of AQP-lmRNA in single lung hypoxia group decreased significantly (P 0.05), and decreased with the increase of hypoxia time, but the expression of AQP-4 did not change significantly. Conclusion: the expression of AQP-1 is down-regulated and negatively correlated with hypoxic metabolic time due to long-term hypoxic metabolism in rats, but AQP-4 has no correlation with hypoxic metabolism. Aim: to study the expression of vascular endothelial growth factor (VEGF) in rat hypoxic metabolism and its correlation. Methods: thirty Sprague-Dawley rats were randomly divided into two groups: control group and experimental group, namely 1 / 2 h one-lung hypoxia group, 1 / 2 h single lung hypoxia group, 1 h single lung hypoxia group and 4 h single lung hypoxia group, the control group was treated with tracheotomy and intubation to perform double lung ventilation. The model of acute hypoxic lung injury was established by tracheotomy and intubation in the experimental group. Lung tissue and serum of the non-ventilated side were taken after 1 / 2 h / 1 h and 2 h / 4 h of ventilation respectively. The pathological changes of lung tissue were observed under light microscope. The content of sE- selectin in serum, VEGF in lung tissue and serum were measured by ELISA method. The expression and distribution of VEGF protein were observed by immunohistochemistry. The expression of VEGFmRNA in rats was measured by real-time fluorescence quantitative PCR. Results with the prolongation of hypoxic time, the blood capillaries of lung tissue in the experimental group were congested, the cavity was filled with more red blood cells, the alveolar septum widened, and the inflammatory cells infiltrated. The expression of sE- selectin in the serum of rats with single lung hypoxia for 1 h or 2 h was significantly higher than that in the control group (P 0.05), and the expression of sE-selectin in the serum of rats with single lung hypoxia for 1 h or 2 h was significantly higher than that in the control group, and the expression of sE-selectin was prolonged with time. The expression of VEGFin in lung tissue of single lung hypoxia group for 4 hours was significantly lower than that in normal group, but the expression in serum of this group was significantly higher than that in control group. The expression of VEGF in lung tissue was observed by immunohistochemistry with the prolongation of hypoxic time. The expression of VEGF mRNA in single lung hypoxia group for 4 h was significantly lower than that in control group (P 0.05), and the expression of VEGF mRNA decreased with the prolongation of hypoxia time. Conclusion: Long-term hypoxic metabolism leads to the changes of lung tissue structure in rats, and the expression of sE- selectin is up-regulated and positively correlated with hypoxic metabolic time, while VEGF is negatively correlated with hypoxic metabolic time.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363
【相似文献】
相关期刊论文 前10条
1 高勇,肖庆隆,沈华;开胸手术术中和术后氧代谢的变化[J];中华麻醉学杂志;2002年05期
2 马利;黄中华;;急性高容量血液稀释对脑氧代谢的影响[J];中国实用医药;2008年28期
3 许继文;马平;;小儿先天性心脏病手术期间组织氧代谢的研究[J];山西医药杂志;2010年02期
4 许幸;脑氧代谢监测的若干进展[J];国外医学.麻醉学与复苏分册;1997年03期
5 曾健生,樊寻梅;氧代谢的监测及临床意义[J];中国实用儿科杂志;2001年07期
6 吴恒义,池丽庄;严重创伤病人氧代谢的特点和监测[J];创伤外科杂志;2002年03期
7 朱雁鸿;罗家坤;董绍兴;杨梅;;失血性休克患者术中氧代谢监测与调控[J];徐州医学院学报;2006年02期
8 吴恒义,白涛,程多今,苏磊,邢锐,杨自力;氧代谢监测在严重创伤治疗中的应用[J];中华创伤杂志;1997年01期
9 赵良,陈学云,景炳文;肺的气体交换和氧代谢[J];急诊医学;1999年02期
10 邵素凤,曹一飞;21例多脏器功能障碍综合征患者氧代谢分析[J];苏州大学学报(医学版);2002年06期
相关会议论文 前10条
1 朱雁鸿;罗家坤;晏宏;;失血性休克病人术中氧代谢监测与调控[A];玉溪市第十四届外科麻醉科学术年会论文摘要汇编[C];2004年
2 酒谷薰;;光学氧代谢测量在中医学研究领域的应用[A];第六次全国中西医结合血瘀证及活血化瘀研究学术大会论文汇编[C];2005年
3 任在方;史载祥;史载祥;张思迅;张久亮;;外伤性SAH病人脑血氧代谢的变化及大蒜素对其的影响[A];第五次全国中西医结合血瘀证及活血化瘀研究学术大会论文汇编[C];2001年
4 秦宗和;方玉明;茆勇;;红细胞压积对创伤并发肺损伤患者氧代谢的影响[A];2009年全国危重病急救医学学术会议论文汇编[C];2009年
5 任在方;史载祥;顾建文;张思迅;张久亮;;外伤性SAH病人脑血氧代谢的变化及大蒜素对其的影响[A];全国中西医结合治疗心血管病及血瘀证高级论坛和研修班论文汇编[C];2004年
6 王若帆;潘清;宁钢民;;微循环氧代谢功能数学模型研究[A];中国微循环学会2014年全国学术会议大会汇编[C];2014年
7 张冬梅;郎淑慧;徐向辉;朱立宏;常业恬;;高容血液稀释联合控制性降压对老年患者循环和氧代谢的影响[A];2009年西部麻醉学术论坛论文汇编[C];2009年
8 秦宗和;方玉明;茆勇;;红细胞压积对创伤并发肺损伤患者氧代谢的影响[A];第七届全国创伤学术会议暨2009海峡两岸创伤医学论坛论文汇编[C];2009年
9 刘长文;陆骏;郑永科;朱克毅;胡炜;;持续高容量血液滤过对急性呼吸窘迫综合症血液动力学和氧代谢的影响[A];2005年浙江省危重病学学术年会论文汇编[C];2005年
10 孙存普;丛建波;陈虎;吴可;王丽萍;段绍瑾;忻文娟;张建中;郑增德;冯桂兰;;癌细胞氧代谢的ESR研究[A];第六届全国波谱学学术会议论文摘要集[C];1990年
相关硕士学位论文 前10条
1 陶文辉;不同Narcotrend指数下老年患者全麻术中脑氧代谢的比较[D];安徽医科大学;2016年
2 段雪静;麻醉的药物—神经效应建模及脑氧代谢研究[D];燕山大学;2016年
3 李琰;不同水平PaCO_2对老年患者脑氧代谢和术后认知功能的影响[D];青岛大学;2016年
4 葛万运;HIF-1α及下游因子GLUT-1、VEGF、EPO在肺低氧代谢中的表达作用机制研究[D];广西医科大学;2012年
5 杨建;AQP-1、AQP-4、VEGF、sE-选择素在肺低氧代谢中的表达[D];广西医科大学;2012年
6 马利;急性高容量血液稀释对脑氧代谢的影响[D];广西医科大学;2002年
7 马骏;急性高血压脑出血患者脑糖氧代谢变化及意义[D];浙江大学;2014年
8 彭永保;控制性低中心静脉压对小肠氧代谢影响的研究[D];广西医科大学;2007年
9 谢玉莲;神经外科手术中患者体温与脑氧代谢的变化及其相关性分析[D];昆明医科大学;2014年
10 苌恩强;HIF-1α、TGF-β1在肺低氧代谢中的表达及作用机制研究[D];广西医科大学;2011年
，本文编号：1542110
本文链接：https://www.wllwen.com/xiyixuelunwen/1542110.html
