二乙基亚硝胺联合四氯化碳诱导原发性肝癌小鼠模型的方法

发布时间：2018-02-27 23:43

本文关键词： 原发性肝癌模型二乙基亚硝胺四氯化碳 CBL/J　出处：《中国新药杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的:探讨原发性肝癌小鼠模型建立的新方法及其可能机制。方法:取60只C57BL/6J小鼠,随机分为正常组(20只)、模型组(40只),每组均雌雄各半。除正常组外,模型组小鼠在出生d 14,腹腔注射二乙基亚硝胺(DEN)2 mg·kg~(-1),并在给药2周后,腹腔注射四氯化碳(CCl4)5 m L·kg~(-1),20%,每周2次共16周。于造模第20周处死小鼠,分离肝脏并称重。紫外-分光光度法检测各组血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性、碱性磷酸酶(ALP)以及甲胎蛋白(AFP)水平,HE染色法观察肝脏病理学改变。结果:与正常组相比,模型组小鼠血清中ALT,AST,ALP,AFP,肝脏指数和结节数显著增加(P0.01)。同时,模型组小鼠共死亡3只,存活的37只中有33只发生肝癌,诱癌成功率为82.5%(33/40);正常组小鼠肝脏未发现明显异常。结论:DEN联合CCl4可成功诱导出小鼠原发性肝癌模型,为肝癌的研究提供了一种新的造模途径。
[Abstract]:Objective: to explore a new method and its possible mechanism for establishing mouse model of primary liver cancer. Methods: sixty C57BL / 6J mice were randomly divided into normal group (n = 20) and model group (n = 40). Mice in the model group received intraperitoneal injection of diethylnitrosamine 2 mg 路kg ~ (-1) ~ (-1) by intraperitoneal injection on day 14, and CCL _ (4) (5 mL 路kg ~ (-1)) ~ (-1) by intraperitoneal injection of CCL _ (4) (5 mL 路kg ~ (-1)) for 16 weeks. Mice were killed at the 20th week of the model. The activity of alanine aminotransferase (alt) and aspartate aminotransferase (AST) in serum of each group were determined by UV spectrophotometry. Alkaline phosphatase (ALP) and AFP) levels were used to observe the pathological changes of the liver. Results: compared with the normal group, the ALT ASTX ALPAFP, the liver index and the number of nodules in the model group were significantly higher than those in the normal group. At the same time, 3 mice died in the model group. 33 of the 37 surviving mice had liver cancer, the successful rate of inducing cancer was 82.5% / 40%, and no obvious abnormality was found in the normal group. Conclusion the mouse primary liver cancer model can be successfully induced by CCl4 combined with CCl4, which provides a new model for the study of liver cancer.
【作者单位】：安徽医科大学临床药理研究所抗炎免疫药物教育部重点实验室抗炎免疫药物安徽省工程技术研究中心;
【分类号】：R-332;R735.7

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