宁波市耐三代头孢菌素志贺菌携带β-内酰胺酶与基因分型

发布时间：2018-02-27 20:48

本文关键词： 志贺菌耐三代头孢菌素超广谱β-内酰胺酶基因分型　出处：《中国人兽共患病学报》2017年06期 　论文类型：期刊论文

【摘要】：目的了解本市耐三代头孢菌素志贺菌携带ESBLs及其基因型,为疾病防控提供依据。方法采用直接和增菌培养法,分离患者标本中志贺菌;药敏采用K-B法,ESBLs志贺菌表型确证采用纸片法;CTX-M、OXA、TEM和SHV耐药基因采用PCR法;耐药基因分型采用核苷酸序列法,用BLAST分析比较确定基因型别。结果药敏筛检出69株耐三代头孢菌素志贺菌,占ESBLs志贺菌的74.19%。检出CTX-M(CTX-M-1群和CTX-M-9群)、OXA和TEM耐药基因,检出率分别为79.71%、79.01%和26.09%,未检出CTX-M-2群和SHV耐药基因。DNA序列比对CTX-M-1群以CTX-M-15型为主,还检出7个其它型;CTX-M-9群则以CTX-M-14型多,其它型检出6个;49株OXA和18株TEM耐药基因测序后均为1型(OXA-1型和TEM-1型)。携带2种以上耐药基因的志贺菌21株,占30.43%。结论本市志贺菌对头孢曲松等三代头孢菌素耐药率较高,检出的ESBLs酶型种类多,CTX-M(CTX-M-1群的CTX-M-15型和CTX-M-9群的CTX-M-14型)是主流酶型,且可同时携带多种耐药基因,给疾病防控带来困难。OXA-1型的高携带率,提示我们应加强分析。B群志贺菌无论是表型的耐药性,还是耐药基因检出率均高于D群志贺菌,该发现有助志贺菌的扩散与流行研究。
[Abstract]:Objective to study the ESBLs and its genotypes of Shigella cephalosporin resistant to the third generation in order to provide the basis for disease prevention and control. Methods Shigella was isolated from patient samples by direct and bacteriostasis culture. Phenotypic confirmation of Shigella Shigella strains by K-B method. Tem and PCR were used for identification of CTX-MX OXAX Tem and SHV resistance genes, and nucleotide sequence method was used for genotyping of drug resistance genes. Results 69 strains of Shigella cephalosporin resistant to third generation were detected by BLAST analysis, which accounted for 74.19% of ESBLs Shigella. OXA and TEM resistant genes of CTX-M(CTX-M-1 group and CTX-M-9 group were detected. The detectable rate was 79.01% and 26.09%, respectively. There was no CTX-M-2 group and SHV resistance gene. The majority of CTX-M-1 group was CTX-M-15 type, and the other 7 other CTX-M-9 strains were CTX-M-14 type, and the positive rate was 79.01% and 26.09%, respectively, and the positive rate was 79.01% and 26.09%, respectively. Six strains of OXA and 18 strains of TEM resistance genes were identified as type 1, OXA-1 and TEM-1. 21 strains of Shigella with more than 2 resistant genes were found to be 30.43. Conclusion Shigella has a higher resistance rate to ceftriaxone and other three generations of cephalosporins. The CTX-M-15 type and CTX-M-14 type of CTX-MN CTX-M-1 group detected by ESBLs are mainstream enzyme types, and can carry multiple drug resistance genes at the same time, which makes it difficult to prevent and control diseases. OXA-1 type has a high carrying rate. It is suggested that we should strengthen the analysis of Shigella spp. B whether it is phenotypic resistance or the detection rate of resistance genes is higher than that of group D Shigella. This finding is helpful to the study of the spread and prevalence of Shigella.
【作者单位】：宁波市疾病预防控制中心;宁波市第二医院;
【基金】：宁波市重大(重点)项目(No.2013C51014) 宁波市优秀中青年卫生技术人才项目(No.2011145)联合资助~~
【分类号】：R378

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