抵抗素介导AMPK信号通路对HepG2细胞脂质代谢的影响

发布时间：2018-03-01 10:41

本文关键词： 抵抗素游离脂肪酸脂质 AMPK HepG细胞　出处：《实用医学杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的:探讨抵抗素对Hep G2细胞脂质代谢的影响及其与腺苷酸活化蛋白激酶(AMPK)通路的关系。方法:50 ng/m L抵抗素及0.5 mmol/L的棕榈酸作用Hep G2细胞,利用si RNA技术抑制Hep G2细胞AMPKα2亚基表达,采用实时荧光定量RT-PCR技术检测脂质代谢相关基因的m RNA水平,用蛋白印迹技术检测AMPK蛋白磷酸化(P-AMPK-Thr172)水平及乙酰辅酶A羧化酶(ACC)蛋白磷酸化(P-ACCSer79)水平,尼罗红(Nile Red)染色后激光共聚焦显微镜检测胞内脂质含量。结果:在基础及胰岛素刺激条件下,抵抗素增加ACC2 m RNA的表达水平及胞内脂质含量(P0.05),降低AMPKα2及肝脂肪酶(HL)m RNA的表达、AMPK及ACC磷酸化水平(P0.05),对ACC1 m RNA表达无影响(P0.05)。结论:抵抗素可通过AMPK途径影响脂质代谢:脂肪酸合成增加、抑制三酰甘油分解,导致Hep G2肝细胞胞内脂质积聚。
[Abstract]:Aim: to investigate the effect of resistin on lipid metabolism in Hep G2 cells and its relationship with adenylate activated protein kinase (AMPK) pathway. Si RNA technique was used to inhibit the expression of AMPK 伪 2 subunit in Hep G2 cells. The m RNA level of genes related to lipid metabolism was detected by real-time fluorescence quantitative RT-PCR. The levels of phosphorylated P-AMPK-Thr172 and phosphorylated P-ACCSer79 of acetyl-coA carboxylase were detected by Western blot. The intracellular lipid content was detected by laser confocal microscopy after Nile Red) staining. Results: under basic and insulin-stimulated conditions, Resistin increased the expression level of ACC2 m RNA and intracellular lipid content (P0. 05%), decreased the expression of AMPK 伪 2 and hepatic lipase halim RNA (P0. 05), but had no effect on ACC1 m RNA expression (P 0. 05). Conclusion: resistin can affect lipid substitution via AMPK pathway. Thanks: fatty acid synthesis increases, Inhibition of triglyceride decomposition resulted in intracellular lipid accumulation in Hep G2 hepatocytes.
【作者单位】：中山大学孙逸仙纪念医院检验科;中山大学孙逸仙纪念医院内分泌科;
【基金】：国家自然科学基金资助项目(编号:30570886) 教育部留学回国人员科研启动基金资助项目(编号:教外司留[2015]1098号)
【分类号】：R3411

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