IL-12p40与IL-12p70在单病原菌性血流感染小鼠模型中的表达及意义

发布时间：2018-03-02 09:13

本文关键词： 血流感染小鼠模型白细胞介素-p 白细胞介素-p　出处：《中华医院感染学杂志》2017年20期 　论文类型：期刊论文

【摘要】：目的探讨白细胞介素-12p40(IL-12p40)和IL-12p70在不同病原菌致小鼠血流感染模型中的动力学变化及意义。方法建立金黄色葡萄球菌、粪肠球菌、大肠埃希菌和肺炎克雷伯菌标准菌株的CD-1(ICR)小鼠血流感染模型并评价;采用蛋白液相芯片检测技术测定各实验组与磷酸缓冲溶液(PBS)的对照组0.5、1、3、6、12、24和48h的IL-12p40和IL-12p70浓度。结果金黄色葡萄球菌组与对照组相比,IL-12p40的浓度稍升高,而粪肠球菌组、大肠埃希菌组和肺炎克雷伯菌组感染后1h即明显上升,3h时达峰值,分别为(216.36±50.63)pg/mL、(118.32±9.98)pg/mL和(129.19±27.11)pg/mL。感染12h后仍维持较高水平,与对照组相比差异有统计学意义(P0.05);金黄色葡萄球菌组IL-12p70浓度在感染后6h时明显升高且达峰值,为(389.31±118.07)pg/ml;粪肠球菌组IL-12p70浓度在感染后3h明显升高,与对照组相比差异有统计学意义(P0.05),大肠埃希菌组与肺炎克雷伯菌组IL-12p70浓度于感染后3h达峰值,分别为(42.20±3.54)pg/mL和(62.80±8.37)pg/mL但升高幅度显著低于金黄色葡萄球菌组和粪肠球菌组(P0.05)。结论 IL-12p40和IL-12p70在四组单病原菌性血流感染模型早期均明显升高,感染金黄色葡萄球菌或粪肠球菌后IL-12p70浓度的升高程度明显高于大肠埃希菌或肺炎克雷伯菌感染。联合检测IL-12p40和IL-12p70等指标对区分革兰阴阳性菌感染有一定价值。
[Abstract]:Objective to investigate the dynamic changes and significance of interleukin-12p40 IL-12p40 (IL-12p40) and IL-12p70 in blood stream infection model of mice induced by different pathogens. Methods Staphylococcus aureus and Enterococcus faecalis were established. Model of blood stream infection in mice with standard strains of Escherichia coli and Klebsiella pneumoniae; The concentrations of IL-12p40 and IL-12p70 in each experimental group and control group with phosphoric acid buffer solution were measured by protein liquid chip assay at 24 and 48 hours. Results the concentration of IL-12p40 in Staphylococcus aureus group was slightly higher than that in control group, while that in Enterococcus faecalis group was higher than that in Staphylococcus aureus group. In Escherichia coli group and Klebsiella pneumoniae group, the peak value was 216.36 卤50.63 g / mL and 129.19 卤27.11 渭 g / mL respectively at 3 h after infection. Compared with the control group, the concentration of IL-12p70 in Staphylococcus aureus group increased significantly and reached its peak at 6 h after infection, which was 389.31 卤118.07 mg / ml, while the concentration of IL-12p70 in Enterococcus faecalis group increased significantly at 3 h after infection. The concentration of IL-12p70 in Escherichia coli group and Klebsiella pneumoniae group reached the peak at 3 h after infection. It was 42.20 卤3.54 ng / mL and 62.80 卤8.37 ng / mL, respectively, but the increase was significantly lower than that in Staphylococcus aureus group and Enterococcus faecalis group (P 0.05). Conclusion IL-12p40 and IL-12p70 increased significantly in the early stage of single pathogen blood stream infection in the four groups. The concentration of IL-12p70 after infection with Staphylococcus aureus or Enterococcus faecalis was significantly higher than that with Escherichia coli or Klebsiella pneumoniae, and the combined detection of IL-12p40 and IL-12p70 had some value in differentiating Gram-positive infection.
【作者单位】：解放军总医院临床检验科;温州医科大学检验医学院生命科学学院;
【基金】：国家重大科学仪器专项基金资助项目(2012YQ18011708)
【分类号】：R-332;R51

【相似文献】