miRNA-140-5p在人骨髓间充质干细胞成脂分化中的表达及其靶基因的预测

发布时间：2018-03-02 09:58

本文关键词： 人骨髓间充质干细胞成脂分化 mi RNA--p 白血病抑制因子受体　出处：《南方医科大学学报》2017年02期 　论文类型：期刊论文

【摘要】：目的筛选出人骨髓间充质干细胞(h MSCs)成脂分化中具有潜在价值的mi RNA及其靶基因,为人类成骨细胞与脂肪细胞分化平衡调控机制的深入研究提供有力实验依据。方法收集h MSCs成脂分化过程中的0、7、14、21 d的细胞样品,应用mi RNA芯片和转录组测序(m RNA-seq)技术,分析4个点细胞中mi RNA和m RNA的表达水平,并将mi RNA与m RNA的表达趋势进行关联分析,聚焦具有研究价值的mi RNA与m RNA相互调控关系,同时,采用Target Scan、Pic Tar和mi Randa等数据库进行靶基因的预测。结果发现mi R-140-5p在成脂分化中表达量呈逐渐下降的趋势,而白血病抑制因子受体(LIFR)表达量呈逐渐升高趋势,二者呈现负调控的对应关系。同时经3个靶基因数据库预测,LIFR为mi R-140-5p共有的靶基因。结论 mi RNA-140-5p可能与人骨髓间充质干细胞的成脂分化密切相关,且通过调控其靶基因LIFR的表达而参与成脂分化。
[Abstract]:Objective to screen the potential value of mi RNA and its target gene in adipogenic differentiation of human bone marrow mesenchymal stem cells (MSCs). Methods Human osteoblasts and adipocytes were collected for 21 days during the adipogenic differentiation of human osteoblasts and adipocytes, and the cell samples were collected for 21 days. Mi RNA microarray and transcriptome sequencing mRNA-seq) technique were used to study the mechanism of differentiation of human osteoblasts and adipocytes. The expression level of mi RNA and m RNA in four point cells was analyzed, and the expression trend of mi RNA and m RNA was analyzed. The relationship between mi RNA and m RNA was focused on, and the relationship between mi RNA and m RNA was also studied. The results showed that the expression of miR-140-5p decreased gradually in adipogenic differentiation, while the expression of leukemia inhibitory factor receptor (LIFR) increased gradually by using the databases such as Target Scano Pic Tar and mi Randa, and the results showed that the expression level of miR-140-5p decreased gradually in adipogenic differentiation, while the expression of leukemia inhibitory factor receptor (LIFR) increased gradually. At the same time, the LIFR was predicted to be a target gene shared by mi R-140-5p by three target gene databases. Conclusion mi RNA-140-5p may be closely related to the adipogenic differentiation of human bone marrow mesenchymal stem cells. Moreover, it participates in adipogenic differentiation by regulating the expression of its target gene LIFR.
【作者单位】：江西省系统生物医学重点实验室;江西省九江学院基础医学院病原生物学教研室;江西省九江学院附属医院临床技能中心;江西省南昌大学附属九江医院内分泌科;
【基金】：国家自然科学基金(81460221,81260283,81160113) 江西省自然科学基金(20161BAB205197)~~
【分类号】：R329.2

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