MicroRNA研究热点的分析

发布时间：2018-03-02 10:32

本文关键词： MicroRNA 研究热点内容分析文献计量学分析　出处：《北京协和医学院》2012年硕士论文　论文类型：学位论文

【摘要】：鉴于microRNA广泛参与发育、病毒防御、造血过程、器官形成、细胞增殖和凋亡、脂肪代谢等过程的调控,与生物的生长、发育及各种疾病进程尤其与癌症密切相关,对microRNA研究热点开展分析具有重要意义。本论文运用定量和定性相结合的研究方法,确定microRNA研究热点,并对研究热点进行深入分析,以了解microRNA研究热点的现状,为科研人员和相关决策制定者提供参考信息。确定的nicroRNA研究热点包括：microRNA研究种类及其调控通路、疾病类别及诊断和治疗、microRNA的治疗应用、microRNA与干细胞研究、nicroRNA相关研究技术。通过对研究热点分析发现,miR-21、let-7和miR-155是重点研究的microRNA种类；肿瘤是nicroRNA相关疾病研究的主要内容,相关肿瘤研究主要集中于乳腺癌、肺肿瘤、肝癌、前列腺癌和白血病；非肿瘤疾病的研究以心血管疾病和代谢性疾病为主要内容；胚胎干细胞、IPS细胞和肿瘤干细胞是microRNA研究中最常见干细胞种类；microRNA常作为疾病尤其是肿瘤诊断的分子标志物；microRNA和寡核苷酸类最常应用于预防和治疗疾病；microRNA相关的研究技术以调查和诊断技术为主,治疗相关技术涉及较少。基于microRNA研究热点的分析结果,结合目前我国在医学领域中的一些研究现状及问题,认为未来microRNA的发展将呈现以下特点：microRNA调控网络以及同其它调控因子网络的整合是未来研究的重点；microRNA研究可为肿瘤等疾病的诊断和治疗提供新策略：乳腺癌等肿瘤研究应注重向临床转化；microRNA种类间的共现网络或有助于识别其调控通路；microRNA研究有助于解决干细胞应用中存在的瓶颈问题；我国microRNA的相关研究应强调论文质量和团队合作意识。
[Abstract]:Since microRNA is widely involved in the regulation of development, virus defense, hematopoiesis, organ formation, cell proliferation and apoptosis, fat metabolism and so on, it is closely related to biological growth, development and various disease processes, especially cancer. It is of great significance to analyze the hot spots of microRNA research. In this paper, the quantitative and qualitative research methods are used to determine the hot spots of microRNA research, and the research hotspots are analyzed in order to understand the current situation of microRNA research hotspots. To provide reference information for researchers and relevant decision makers. The research hotspots of nicroRNA include the types of microRNAs and their regulatory pathways, the types of diseases and the therapeutic application of microRNAs for diagnosis and treatment. It was found that miR-21 let-7 and miR-155 were the main types of microRNA, tumor was the main content of nicroRNA related diseases, and the related tumor research was mainly focused on breast cancer, lung tumor, liver cancer, prostate cancer and leukemia. Cardiovascular disease and metabolic disease are the main contents in the study of non-tumor diseases. Embryonic stem cell IPS cells and tumor stem cells are the most common stem cell types in microRNA studies. MicroRNAs are often used as molecular markers for the diagnosis of diseases, especially tumors. MicroRNAs and oligonucleotides are most commonly used in the prevention and treatment of diseases related to microRNAs. The main techniques are investigation and diagnosis. Treatment-related techniques are less involved. Based on the analysis results of microRNA research hot spots, combined with some current research status and problems in the field of medicine in China, It is believed that the future development of microRNA will be characterized by the following characteristics: microRNA regulatory network and integration with other regulatory factor networks are the key points of future research. MicroRNA research can provide new strategies for the diagnosis and treatment of cancer and other diseases: breast cancer and other tumors. The research should focus on the coexisting network between microRNA types or help to identify its regulatory pathways. The study of microRNA can help to solve the bottleneck problems in stem cell application. Our country microRNA related research should emphasize the thesis quality and the team cooperation consciousness.
【学位授予单位】：北京协和医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R346

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