化痰类中药干预肺纤维化模型大鼠肺组织中Nrf2含量的研究

发布时间：2018-03-02 17:00

本文选题：化痰类中药　切入点：肺纤维化　出处：《时珍国医国药》2017年09期 　论文类型：期刊论文

【摘要】：目的探讨部分化痰类药物在博莱霉素所致模型大鼠肺纤维化中的抗氧化作用及途径。方法采用气管内滴入博莱霉素的方法形成大鼠肺纤维化模型,以天南星、川贝母、海藻、胖大海、清半夏、白前、黄药子、前胡等8味中药的水煎液或吡非尼酮对模型大鼠进行灌胃干预,检测各组大鼠肺组织中Nrf2的含量变化。结果 8味中药组、对照组(模型组)、吡非尼酮组大鼠肺组织中的Nrf2含量明显高于空白组(P0.05),川贝组、半夏组、天南星组、海藻组、胖大海组与对照组比较均P0.01,而吡非尼酮组与对照组比较P0.05,差异具有统计学意义。黄药子组、前胡组、白前组等3味中药组与对照组比较均P0.05。之后再将剩余的5味中药组(川贝组、半夏组、天南星组、海藻组、胖大海组)与吡非尼酮组相比较均P0.05。说明上述5味中药在改善Nrf2抗氧化指标及调控抗氧化基因表达方面与吡非尼酮作用相当。结论部分化痰类中药能有效提高模型大鼠肺组织中Nrf2的含量,具有较强的修复细胞及机体解毒能力,其中川贝、半夏、天南星、海藻、胖大海的作用较为突出,证实了部分化痰类中药可以通过干预机体的氧化-抗氧化机制对肺纤维化起到干预作用。
[Abstract]:Objective to investigate the antioxidation effect of some phlegm drugs on pulmonary fibrosis induced by bleomycin in rats. Methods Pulmonary fibrosis model of rats was established by intratracheal instillation of bleomycin. The model rats were treated with the decoction of eight kinds of traditional Chinese medicine, such as fat sea, clear Pinellia ternata, prewhiten, Huangzi, Qianhu, or pifenidone. The changes of Nrf2 content in lung tissue of rats in each group were detected. Results eight kinds of traditional Chinese medicine group were treated with traditional Chinese medicine. The content of Nrf2 in the control group (model group, pifenidone group) was significantly higher than that in the blank group (P 0.05), Chuanbei group, Pinellia ternata group, Tiannan Xing group, and seaweed group. Compared with the control group, the P0.01in the fat sea group, and the P0.05 in the pifenidone group compared with the control group, the difference was statistically significant. Compared with the control group, P0.05. the remaining five Chinese medicine groups (Chuanbei group, Pinellia ternata group, Tiannan Xing group, seaweed group) were added. Compared with pifenidone group, the P0.05. the results show that the above five herbs have the same effect in improving the antioxidant index of Nrf2 and regulating the expression of antioxidant gene. Conclusion the Chinese herbal medicine of partial phlegm reduction can effectively improve the model. The content of Nrf2 in lung tissue of rats. Has strong repair cells and body detoxification ability, among them Sichuan shellfish, Pinellia ternata, Tiannan star, seaweed, fat sea more prominent role, It is proved that partial phlegm can interfere with pulmonary fibrosis by intervening the oxidation-anti-oxidation mechanism.
【作者单位】：山东中医药大学附属医院;
【基金】：国家自然科学基金(No.81273704)
【分类号】：R285.5;R-332

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