混合皮肤移植中自体角朊细胞表达B7-H1诱导免疫抑制的相关研究

发布时间：2018-03-02 18:05

本文选题：角朊细胞　切入点：B-H　出处：《中国免疫学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的:探讨自体角朊细胞表达的B7-H1分子在混合皮肤移植中的作用和相关机制。方法:体外模拟混合皮肤移植模拟系统(MELC体系),通过流式细胞术检测角朊细胞B7-H1和淋巴细胞PD-1的表达。同时,实时荧光定量PCR检测淋巴细胞IL-10、Foxp3、GATA-3 mRNA的表达。结果:流式细胞仪检测结果显示,在有自体角朊细胞参与的MELC体系中,随着作用时间增加,角朊细胞上B7-H1的表达和淋巴细胞上PD-1的表达水平均明显升高,且具有时间依赖性(P0.01);实时荧光定量PCR检测结果显示,随着作用时间增加,与无自体角朊细胞参与的MELC体系相比,在自体角朊细胞参与的MELC体系中,淋巴细胞IL-10、GATA-3、Foxp3 mRNA的表达水平均明显升高,且具有时间依赖性(P0.01)。结论:在体外混合皮肤移植模拟系统中,自体角朊细胞通过表达共刺激分子B7-H1,上调淋巴细胞表面PD-1的表达,二者相结合,诱导Th2细胞和Foxp3+的Treg细胞分化,从而负向调节免疫反应。
[Abstract]:Objective: to investigate the role and mechanism of B7-H1 molecule expressed by autologous keratinocytes in mixed skin transplantation. Methods: in vitro, the mixed skin transplantation simulation system was used to detect B7-H1 and B7-H1 of keratinocytes by flow cytometry. The expression of PD-1 in lymphocytes. Real-time fluorescence quantitative PCR was used to detect the expression of IL-10 Foxp3 + GATA-3 mRNA in lymphocytes. Results: the results of flow cytometry showed that in the system of MELC with autologous keratinocytes, the expression of IL-10 Foxp3 + GATA-3 was increased with the increase of time. The expression of B7-H1 in keratinocytes and the expression of PD-1 in lymphocytes were significantly increased in a time-dependent manner, and the results of real-time fluorescence quantitative PCR showed that the expression of B7-H1 in keratinocytes was significantly higher than that in MELC systems without autologous keratinocytes, and the expression of B7-H1 in keratinocytes was significantly higher than that in MELC without autologous keratinocytes. In the MELC system with the participation of autologous keratinocytes, the expression level of IL-10, GATA-3, Foxp3 mRNA in lymphocytes was significantly increased, and the expression level was time-dependent. Conclusion: in the mixed skin transplantation simulation system in vitro, the expression of IL-10 + GATA-3T Foxp3 protein was significantly increased in vitro. Autologous keratinocytes induce the differentiation of Th2 cells and Treg cells of Foxp3 by expression of B7-H1 and up-regulate the expression of PD-1 on the surface of lymphocytes.
【作者单位】：福建医科大学附属协和医院检验科;上海市免疫学研究所;
【基金】：国家自然科学基金项目(81171656)
【分类号】：R392

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