少腹逐瘀汤对子宫内膜异位症模型大鼠在位内膜增殖、凋亡及血管生成的影响

发布时间：2018-03-03 03:34

本文选题：少腹逐瘀汤　切入点：子宫内膜异位症　出处：《东南大学学报(医学版)》2017年02期 　论文类型：期刊论文

【摘要】：目的:观察少腹逐瘀汤对子宫内膜异位症模型大鼠在位内膜增殖、凋亡及血管生成的影响,探讨少腹逐瘀汤治疗子宫内膜异位症的机制。方法:58只SD雌性大鼠中随机选择10只作为对照组,剩余大鼠采用自体移植法建立子宫内膜异位症大鼠模型,造模成功的38只大鼠随机分为模型组及治疗组,治疗组给予少腹逐瘀汤灌胃,给药4周。HE染色观察在位内膜组织形态及内膜厚度,免疫组化法检测PCNA表达,TUNEL法检测细胞凋亡,免疫荧光双标法检测CD34、SMA-α表达以评估微血管密度(MVD)及血管成熟指数(VMI)。结果:模型组在位内膜较对照组、治疗组明显增厚(P0.05),在位内膜凋亡指数明显低于对照组及治疗组(P0.01),MVD值明显高于对照组及治疗组(P0.05),VMI值明显低于对照组及治疗组(P0.01)。结论:少腹逐瘀汤可以通过诱导子宫内膜异位症模型大鼠在位内膜间质细胞凋亡来抑制在位内膜增殖,并能够抑制在位内膜血管生成,促进微血管成熟。
[Abstract]:Objective: to observe the effect of Shaofan Zhuyu decoction on the proliferation, apoptosis and angiogenesis of eutopic endometrium in rats with endometriosis. To explore the mechanism of Shaofan Zhuyu decoction in the treatment of endometriosis. Methods Ten of 58 female SD rats were randomly selected as control group, and the remaining rats were used to establish the model of endometriosis by autologous transplantation. Thirty-eight rats were randomly divided into two groups: model group and treatment group. The rats in the treatment group were treated with Shaofan Zhuyu decoction for 4 weeks. The morphology and thickness of eutopic endometrium were observed by HE staining. The expression of PCNA was detected by Tunel and apoptosis was detected by immunohistochemical method. The expression of CD34 SMA- 伪 was detected by immunofluorescence double labeling method to evaluate the microvessel density (MVD) and vascular maturity index (VMIA). Results: the eutopic endometrium in the model group was higher than that in the control group. The apoptotic index of eutopic endometrium in the treatment group was significantly lower than that in the control group and the treatment group. The MVD value of the treatment group was significantly higher than that of the control group and the treatment group. Conclusion: Shaofangzhuyu decoction can induce uterus by inducing uterus. In order to inhibit the proliferation of eutopic endometrium, the eutopic endometrial stromal cells apoptosis in the rat model of endometriosis. It can inhibit the angiogenesis of eutopic endometrium and promote microvascular maturation.
【作者单位】：华北理工大学;华北理工大学附属医院;
【基金】：国家自然科学基金资助项目(81603455) 河北省中医药管理局科研计划项目(2015165) 2015年河北省大学生创新创业训练计划项目 2015年华北理工大学大学生创新创业训练计划项目(X2015061)
【分类号】：R285.5;R-332

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