热毒血瘀证结肠癌荷瘤小鼠模型的建立及清热解毒活血化瘀法对其的影响

发布时间：2018-03-04 14:24

本文选题：病证复合模型　切入点：结肠癌　出处：《中华中医药杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:建立热毒血瘀证荷结肠癌复合动物模型并探索清热解毒活血化瘀法对其影响。方法:用脂多糖(LPS)尾静脉和腹腔注射建立热毒血瘀证模型,C26皮下注射建立荷瘤模型,设空白对照组,单纯荷瘤组,复合模型对照组,复合模型高、中、低剂量组,中药干预后行量化评分表、局部微循环血流量及血液流变学相关指标的检测。结果:复合模型各组造模1周起出现不同程度热毒血瘀证。与复合模型对照组比较,复合模型高、中剂量组造模12-42d量化评分值明显减轻(P0.05,P0.01)。造模后复合模型组局部血流量减低,给药后各剂量组局部血流量增高,以复合模型高剂量组更为明显。造模后,各组全血黏度值均升高明显,运用活血化瘀法后可在一定程度上改善血液的高黏滞状态。结论:本实验建立热毒血瘀证结肠癌的病证复合模型,符合中医证型特点;采用不同剂量的清热解毒活血化瘀中药进行干预,能不同程度减少热毒血瘀的症状和体征。
[Abstract]:Objective: to establish the animal model of heat toxin and blood stasis syndrome combined with colon cancer and to explore the effect of the method of clearing heat, detoxifying, activating blood circulation and removing blood stasis on it. Methods: the model of heat toxin and blood stasis syndrome was established by subcutaneous injection of C26 by injection of lipopolysaccharide into tail vein and intraperitoneally. Set up blank control group, simple tumor bearing group, compound model control group, compound model high, medium and low dosage group, Chinese medicine intervention after the quantitative score table, Results: there were different degrees of heat toxin and blood stasis syndrome in each group from 1 week. Compared with the control group of the compound model, the composite model was higher than that of the control group. The quantitative score value of 12-42 days in the middle dose group significantly reduced the local blood flow in the compound model group, but increased in each dose group, especially in the high dose group, after the model was made, the local blood flow in the compound model group was decreased, especially in the high dose group, after the model was made, the local blood flow was increased in the compound model group, especially in the high dose group after the establishment of the model. The whole blood viscosity of each group was significantly increased, and the blood hyperviscosity could be improved to a certain extent by activating blood circulation and removing blood stasis. Conclusion: this experiment established the complex model of heat toxin and blood stasis syndrome of colon cancer, which accords with the characteristics of TCM syndrome. Different doses of traditional Chinese medicine for clearing heat, promoting blood circulation and removing blood stasis can reduce the symptoms and signs of heat toxin and blood stasis in different degrees.
【作者单位】：浙江中医药大学;浙江中医药大学附属第一医院;浙江中医药大学附属第二医院;浙江中医药大学附属第三医院;
【基金】：浙江省中医药科学研究基金计划(A类)项目(No.2012ZA049) 浙江省自然科学基金一般项目(No.Y13H290038)~~
【分类号】：R285.5;R-332

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