重症肌无力动物模型的建立及中药治疗重症肌无力的机制研究

发布时间：2018-03-04 22:14

本文选题：重症肌无力　切入点：实验性自动免疫性重症肌无力　出处：《吉林大学》2011年博士论文　论文类型：学位论文

【摘要】：本文通过目前得到大家公认的三种方法建立重症肌无力动物模型,经过体重变化、临床症状观察、肌电图及血清乙酰胆碱受体抗体等的检测,经典的通过电鳗电器官提纯乙酰胆碱受体免疫易感鼠雌性健康的C57BL/6J小鼠,成功的建立了重症肌无力动物模型。对造模成功的小鼠给予目的药物(肌强散)治疗,同样喂养条件下给予生理盐水对照和激素治疗,以对比肌强散的疗效,并通过各种特异性细胞因子的研究和病理变化探讨肌强散治疗重症肌无力的机制。通过研究,肌强散对于重症肌无力的治疗有明确的疗效,其大中剂量组与激素组疗效无明显差异。肌强散能升高EAMG小鼠淋巴细胞TGF-β水平,通过抑制B、T淋巴细胞的增殖和分化从而抑制IL-4、IFN-γ等细胞因子的过量分泌,从而降低血清nAchR-Ab的水平,减少nAchR-Ab与nAchR的α亚单位中的MIR结合,减少突触后膜的损伤,从而达到改善EAMG小鼠肌无力症状的作用。本实验能证明肌强散疗效确切,通过调整免疫功能、改善乙酰胆碱受体功能、增强肌肉收缩能力,使MG的症状得到缓解和根治,将为下一步肌强散的临床应用提供理论依据。我们的长期目标是最终使肌强散转化为中成药,它能使那些因路途遥远、行动不便、求生心切的患者得到及时治疗,使陷入求生迷茫的患者早日见到光明,填补我省中医药史上的空白。
[Abstract]:In this paper, the animal model of myasthenia gravis was established by three methods that have been widely accepted. The changes of body weight, the observation of clinical symptoms, the detection of electromyogram (EMG) and the detection of serum acetylcholine receptor antibody, etc. Classical C57BL / 6J mice immunized with acetylcholine receptor (ACCR) were isolated from electric eel and successfully established an animal model of myasthenia gravis. In order to compare the curative effect of Jiqiang Powder, the mice were treated with the target drug (Jiqiang Powder) and the same feeding condition with normal saline and hormone. The mechanism of myasthenia gravis treated by Jiqiangsan was discussed through the study of various specific cytokines and pathological changes. Through the research, Jiqiangsan has definite curative effect on the treatment of myasthenia gravis. There was no significant difference between the high and middle dose group and the hormone group. Jiqiang San could increase the level of TGF- 尾 in lymphocytes of EAMG mice, and inhibit the excessive secretion of cytokines such as IL-4 and IFN- 纬 by inhibiting the proliferation and differentiation of BU T lymphocytes, thereby reducing the level of serum nAchR-Ab. To reduce the binding of MIR in 伪 subunit of nAchR-Ab and nAchR and to reduce the damage of postsynaptic membrane, so as to improve the symptoms of myasthenia in EAMG mice. Improving the function of acetylcholine receptor, enhancing muscle contractility, relieving and curing the symptoms of MG will provide theoretical basis for the clinical application of Jiqiangsan in the next step. Our long-term goal is to ultimately transform Jiqiang Powder into a traditional Chinese patent medicine. It can make those patients who have long journey, inconvenient movement and eager to survive get timely treatment, so that the patients who fall into the confusion of survival can see the light as soon as possible, and fill the blank in the history of traditional Chinese medicine in our province.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2011
【分类号】：R285.5;R-332

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