Sn-PCR在脑脊液中未知肠道病毒鉴定中的应用

发布时间：2018-03-05 05:20

本文选题：肠道病毒　切入点：病毒性脑炎　出处：《中国病原生物学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的利用肠道病毒分子定型试验,对病毒性脑炎患儿的脑脊液标本进行分子型别鉴定。方法提取患儿脑脊液核酸,利用半巢式聚合酶链反应(Sn-PCR)对肠道病毒VP1区进行特异性扩增并进行序列测定,通过BLAST比对确定肠道病毒的类型,采用Mega6.0软件构建基因进化树。结果 BLAST比对显示该病毒是肠道病毒中的柯萨奇病毒B5(Coxsackievirus group B5,CVB5)。从进化树上看,青海分离株04/CVB5/2010/Qinghai/CHN由2009年山东分离株SD2009-067不断演变而来,而与分离株AH-HK19/CHN/2010/CVB5不在同一进化分支,相距甚远,与FJLY/2011096h分离株亲缘性关系较近。结论成功应用Sn-PCR对患儿脑脊液中未知肠道病毒进行鉴定,确定肠道病毒类型是CVB5型,为肠道病毒引起的脑炎疾病的防控提供参考。
[Abstract]:Objective to identify the molecular types of cerebrospinal fluid (CSF) samples of children with viral encephalitis by molecular typing test of enterovirus. Methods the nucleic acids of CSF were extracted from children with viral encephalitis. The VP1 region of enterovirus was amplified and sequenced by semi-nested polymerase chain reaction (Sn-PCR). The type of enterovirus was determined by BLAST comparison. Mega6.0 software was used to construct the gene evolutionary tree. Results the BLAST comparison showed that the virus was coxsackievirus B5Coxsackievirus group B5CVB5. From the evolutionary tree, Qinghai isolate 04 / CVB5 / 2010 / Qinghai / Chn evolved continuously from the Shandong isolate SD2009-067 in 2009. However, the relationship between AH-HK19/CHN/2010/CVB5 and AH-HK19/CHN/2010/CVB5 was not the same evolutionary branch, and the genetic relationship was close to that of FJLY/2011096h. Conclusion Sn-PCR was successfully used to identify unknown enterovirus in cerebrospinal fluid of children, and the type of enterovirus was identified as CVB5 type. It provides reference for the prevention and control of encephalitis caused by enterovirus.
【作者单位】：青海省疾病预防控制中心;
【基金】：国家科技重大专项传染病监测技术平台项目(No.2012ZX10004-208)
【分类号】：R373.2

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