骨髓源间充质干细胞注射疗法对肝硬化大鼠模型免疫系统的调节作用及其相关机制研究

发布时间：2018-03-05 07:09

本文选题：髓间充质干细胞　切入点：肝硬化　出处：《免疫学杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的探讨骨髓间充质干细胞移植治疗对肝硬化大鼠自身免疫功能的影响,为临床治疗方法提供理论依据。方法选取150只健康雄性Wistar大鼠,随机分为3组,即对照组(不采取任何干预措施)、肝硬化组(行大鼠肝硬化模型制备)、治疗组(行大鼠肝硬化模型制备后给予骨髓间充质干细胞移植治疗)。检测各组大鼠脾脏T、B淋巴细胞增殖情况,CD4~+CD25~+Foxp3~+调节性T细胞的水平,Foxp3 mRNA表达水平。结果对照组大鼠肝细胞结构完整,没有纤维增生及假小叶存在;肝硬化组大鼠肝小叶结构破坏,假小叶出现,肝细胞水肿变性坏死,可见炎性细胞浸润;实验组大鼠肝细胞水肿变性坏死程度较肝硬化组显著降低,但仍可见增生的纤维组织。肝硬化组大鼠脾脏T、B淋巴细胞体外增殖情况明显高于对照组(P0.05),经BMSCs治疗后,治疗组大鼠脾脏T、B淋巴细胞体外增殖情况显著低于肝硬化组(P0.05);肝硬化组大鼠脾脏Foxp3 mRNA的表达水平明显低于对照组(P0.05),经BMSCs治疗后,治疗组脾脏Foxp3 mRNA的表达水平高于肝硬化组(P0.05);肝硬化组大鼠脾脏CD4~+CD25~+Foxp3~+调节性T细胞百分比明显低于正常组(P0.05),经BMSCs治疗后,治疗组脾脏CD4~+CD25~+调节性T细胞百分比高于肝硬化组(P0.05)。结论骨髓间充质干细胞移植治疗可通过抑制脾脏T、B淋巴细胞增殖,升高CD4~+CD25~+Foxp3~+调节性T细胞及Foxp3 mRNA表达水平,影响自身免疫功能,从而影响疾病的转归。
[Abstract]:Objective to investigate the effect of bone marrow mesenchymal stem cell transplantation for the treatment effect on immune function in rats with liver cirrhosis, and provide a theoretical basis for clinical treatment. Methods 150 healthy male Wistar rats were randomly divided into 3 groups: control group (no intervention measures), liver cirrhosis group (for liver cirrhosis in rats preparation of the model), treatment group (bone marrow mesenchymal stem cell transplantation for treatment of liver cirrhosis model rats were given after preparation). Detection of spleen of T rats, B lymphocyte proliferation, CD4~+CD25~+Foxp3~+ regulatory T cells, mRNA expression level of Foxp3. The control structure of liver cells in rats, there is no fiber and pseudolobular proliferation; destruction of hepatic lobules of liver cirrhosis rats, pseudolobule hepatic cell edema, necrosis, inflammatory cells infiltration; experimental group rat liver cell edema degeneration and necrosis of liver cirrhosis group was significant Reduced, but still visible hyperplasia of fibrous tissue. The spleen T in cirrhotic rats, B lymphocyte proliferation was significantly higher than the control group (P0.05), after BMSCs treatment, treatment of spleen of rats in the T group, B lymphocyte proliferation was significantly lower than that in cirrhosis group (P0.05); the expression level of hepatic cirrhosis rats spleen Foxp3 mRNA was significantly lower than the control group (P0.05), after BMSCs treatment, the expression level of mRNA is higher than Foxp3 treatment group spleen liver cirrhosis group (P0.05); liver cirrhosis rats spleen CD4~+CD25~+Foxp3~+ percentage of T cells was significantly lower than the normal group (P0.05), after BMSCs treatment, treatment group spleen CD4~+CD25~+ the percentage of T cells higher than the cirrhosis group (P0.05). Conclusion: bone marrow mesenchymal stem cells transplantation through inhibition of spleen T, B lymphocyte proliferation, increased CD4~+CD25~+Foxp3~+ regulatory T cells and Foxp3 mRNA expression level, its influence The immune function, which affects the prognosis of the disease.

【作者单位】：广州卫生职业技术学院检验系;中山大学附属肿瘤医院生物治疗中心;广州市番禺区中心医院皮肤科;
【分类号】：R-332;R575.2

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