肾阳虚外感小鼠模型建立及麻黄细辛附子汤干预研究

发布时间：2018-03-05 13:07

本文选题：肾阳虚外感　切入点：小鼠模型　出处：《山东中医药大学》2012年博士论文　论文类型：学位论文

【摘要】：本论文首先对流感病毒致病特点以及中西医防治流行性感冒（简称流感）研究进展进行总结，针对目前流感防治领域的重点和难点—流感高危人群的防治的重要性进行了专门论述，随后阐述了流感的高危人群多是属于中医的肾阳虚体质，并以《伤寒杂病论》经方为着眼点，总结了麻黄细辛附子汤治疗肾阳虚外感的古今临床应用。在此基础上进行了实验研究，本课题首次建立了肾阳虚外感病证动物模型，并用麻黄细辛附子汤对其干预，初步探讨了麻黄细辛附子汤防治该病证的作用机理。目的：通过病证结合的办法，采用复合手段造模，建立了肾阳虚外感动物模型，利用该模型，观察麻黄细辛附子汤对病证模型的影响，初步探讨麻黄细辛附子汤干预肾阳虚外感的作用机制。方法：采用雌二醇腹腔注射复制肾阳虚小鼠模型，在此基础上鼻腔滴注流感病毒FM1建立了肾阳虚外感病证模型，应用麻黄细辛附子汤对该模型进行干预，比较动物一般状况、生理状态、肺指数、死亡率等评价麻黄细辛附子汤对肾阳虚外感模型的防治作用，并从细胞因子TNF-α、IFN-γ和抗氧化能力SOD、MDA水平初步探讨麻黄细辛附子汤防治肾阳虚外感作用机理。结果：1.通过对小鼠一般状况、生理状态、肺指数、肺组织病理切片等观察，表明本实验成功的建立了肾阳虚外感病证模型。模型小鼠精神状态低迷、趾温、肛温显著性降低，死亡率升高，肺组织病理改变明显。2.麻黄细辛附子汤干预后可明显改善病证模型动物的生理状况，显著性升高体温，降低死亡率，TNF-α水平显著性升高，肺组织免疫病理损伤降低。3.麻黄细辛附子汤干预后可明显增加动物的抗氧化能力，升高动物血清当中的SOD含量，降低MDA水平。结论：雌二醇腹腔注射结合流感病毒FM1滴注可成功的建立肾阳虚外感小鼠病证模型，，麻黄细辛附子汤可明显改善肾阳虚外感动物的生理状况，降低肺组织的病理损伤，显著性升高血清中TNF-α水平，增强机体的抗氧化能力，从而达到对该病证的防治作用。
[Abstract]:This paper first summarizes the pathogenic characteristics of influenza virus and the progress in the prevention and treatment of influenza by traditional Chinese and western medicine. The importance of prevention and treatment of high risk groups of influenza in the field of influenza prevention and treatment at present is specially discussed. Then, it is expounded that most of the high-risk groups of influenza belong to the kidney-yang deficiency constitution of traditional Chinese medicine. The clinical application of ephedra Asarum aconite decoction in the treatment of kidney yang deficiency was summarized. Based on the experimental study, the animal model of kidney yang deficiency syndrome was established for the first time. The mechanism of ephedra Asarum aconite decoction in preventing and treating the disease syndrome was discussed. Objective: to establish an animal model of kidney yang deficiency by combining disease with syndromes, and to observe the effect of ephedra Asarum aconite decoction on the model of disease syndrome. Objective: to explore the mechanism of ephedra Asarum aconite decoction in the treatment of kidney yang deficiency. Methods: the mouse model of kidney yang deficiency was induced by intraperitoneal injection of estradiol, and the model of syndrome of kidney yang deficiency was established by nasal drip of influenza virus FM1. The model was treated with ephedra Asarum aconite decoction, and the general condition of animals was compared. Physiological status, lung index and mortality were evaluated to evaluate the preventive and therapeutic effects of ephedra Asarum aconite decoction on the exogenous model of kidney yang deficiency, and the mechanism of ephedra Asarum aconite decoction in preventing and treating kidney yang deficiency was preliminarily discussed from the levels of cytokine TNF- 伪, IFN- 纬 and antioxidant capacity (SODN MDA). Results 1. By observing the general condition, physiological state, lung index and pathological section of lung tissue in mice, the experimental results showed that the model of kidney yang deficiency was successfully established. The mental state of the model mice was low, the toe temperature and anal temperature were significantly decreased. After intervention of ephedrine Asarum aconite decoction, the physiological status of the model animals was improved, the body temperature was significantly increased, and the death rate was significantly decreased, and the level of TNF- 伪 was significantly increased. The immune pathological damage of lung tissue was decreased .3.After the intervention of Asarum ephedra decoction, the antioxidant ability of animals was increased, the content of SOD in serum was increased, and the level of MDA was decreased. Conclusion: intraperitoneal injection of estradiol combined with FM1 drip of influenza virus can successfully establish the model of kidney yang deficiency and exogenous disease in mice. Ephedra Asarum aconite decoction can obviously improve the physiological condition and reduce the pathological injury of lung tissue in the exogenous animals with deficiency of kidney yang. The level of TNF- 伪 in serum was significantly increased, and the antioxidant capacity of the body was enhanced, so that the prevention and treatment of the disease could be achieved.
【学位授予单位】：山东中医药大学
【学位级别】：博士
【学位授予年份】：2012
【分类号】：R-332;R285.5

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