过度运动可引起大鼠冈上肌腱损伤和腱细胞凋亡

发布时间：2018-03-05 13:40

本文选题：过度运动　切入点：细胞凋亡　出处：《新疆医科大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的：探讨过度运动对大鼠冈上肌腱组织和腱细胞的影响。方法：采用40只清洁级Sprague-Dawley大鼠随机均分为8组,运动组7组,对照组1组,每组5只。运动组以17m/s,下倾10°于跑台训练,每天1小时,每周5天建立冈上肌腱过度运动模型。六组运动组于训练1W、2W、3W、4W、5W、6W后麻醉下取冈上肌腱；另一组训练6周完毕,再于笼内休息2周后麻醉下取材,视为8W运动组。对照组于笼内自由活动,视为0W组。HE观察组织病理变化。通过免疫组化TUNEL法检测腱细胞凋亡和天冬氨酸特异性半胱氨酸蛋白酶3(Caspase 3)的活化情况。显微镜下计数凋亡细胞指数统计分析,取α=0.05为假设检验水准。结果：(1)HE染色可见3W、4W组出现肌腱内软骨样细胞数目增多,排列不规则。5W、6W组出现肩袖止点潮线消失结构,软骨细胞增生。8W运动组可见潮线改变恢复不明显,潮线结构和矿化的纤维软骨结构分界不清晰。(2)对照组仅有较低的凋亡水平。1W至4W组渐增,TUNEL和Caspase 3活化表达的凋亡率与对照组相比差异无统计学意义。5W、6W组与0W组相比两者有统计学意义(P0.05),Caspase 3阳性细胞指数5 W达高峰,TUNEL凋亡指数6W达高峰。8W组与0W组相比细胞凋亡和Caspase 3活化表达差异均有统计学意义(P0.05),而与5W、6W组相比两者均无统计学意义(P0.05)。结论：过度运动可以导致大鼠冈上肌腱损伤,使肌腱止点处潮线结构发生退行性变以及腱细胞发生凋亡和Caspase 3活化表达的增高。
[Abstract]:Objective: To investigate the influence of excessive exercise on rat supraspinatus tendon and tendon cells. Methods: 40 Sprague-Dawley rats were randomly divided into 8 groups, group 7 groups, 1 control groups, 5 rats in each group. Exercise group with 17m/s, declined 10 degrees in treadmill training, 1 hours a day 5 days a week, the establishment of the supraspinatus tendon excessive motion model. The six group exercise group in 1W training, 2W, 3W, 4W, 5W, 6W after anesthesia in the supraspinatus tendon; another group after 6 weeks of training, and then on the cage rest for 2 weeks after anesthesia were treated as control group. 8W in cage activity, as the observation of pathological changes in 0W group..HE were detected by TUNEL immunohistochemical staining of tendon cell apoptosis and caspase 3 (Caspase 3) activation. Analysis of apoptotic cells was counted under the microscope statistical index, take a =0.05 for the level of hypothesis test. Results: (1) HE staining, 3W, 4W Group number of chondrocytes within the tendon increased, irregular arrangement of.5W, 6W group of rotator cuff tidal line structure disappeared, cartilage cell proliferation.8W movement group showed no obvious recovery to change the tide line, boundary line structure and the structure of the tide of cartilage mineralization is not clear. (2) control group.1W apoptosis level to 4W only low increasing group, TUNEL and Caspase 3 expression of apoptosis rate compared with the control group there was no significant difference in.5W, 6W group and 0W group was statistically significant compared with both (P0.05), the Caspase 3 index of positive cells reached the peak at 5 W, TUNEL apoptosis index peaked at 6W in.8W group compared with 0W group, apoptosis 3 activation and Caspase expression differences were statistically significant (P0.05), and 5W, 6W group both had no statistical significance (P0.05). Conclusion: excessive exercise can lead to injury of rat supraspinatus tendon, the tendon at the tide line structure to neurodegeneration. The apoptosis of the tendon cells and the increased expression of Caspase 3 were increased.

【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363

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