基于COI基因片段的拟钉螺种类鉴定初探

发布时间：2018-03-06 08:17

本文选题：拟钉螺亚科　切入点：COI基因　出处：《中国人兽共患病学报》2017年08期 　论文类型：期刊论文

【摘要】：目的探讨COI基因片段在拟钉螺种类鉴定中的作用。方法收集来自福建省内多个地区的疑似拟钉螺标本,用形态学方法进行物种鉴定后,取出螺肉提取基因组DNA,通过PCR的方式扩增COI基因序列片段并测序,通过序列比对及系统进化分析鉴定其物种。结果共采集到标本13份,通过形态学方法将样本鉴定为福建境拟钉螺属。所有标本经实验扩增到长度为515~598bp的COI基因片段。13份标本中11株的COI基因片段与γ拟钉螺属最为相似(88.96%~97.82%),另外2株分别与拟钉螺属下的武鸣拟钉螺(87.08%)及新拟钉螺属下的开放新拟钉螺(88.55%)最为相似。在属水平上,13份标本中仅有1份的形态学鉴定结果与基因鉴定结果吻合,其余12份样本的形态学鉴定结果均与基因鉴定不同,两种方法的鉴定结果存在较大差异。结论通过COI基因检测可快速对拟钉螺亚科作出准确判断,但对属水平的鉴定的准确性还需要进一步的研究。
[Abstract]:Objective to investigate the role of COI gene fragment in the species identification of Oncomelania hupensis. Genomic DNA was extracted from snail meat, COI gene sequence was amplified by PCR and sequenced. The species was identified by sequence alignment and phylogenetic analysis. The samples were identified as the genus Oncomelania hupensis by morphological method. The COI gene fragments of 11 of the 13 samples of 515598 BP in length were amplified by experiments. The COI gene fragment of 11 strains was the most similar to that of the genus 纬 Oncomelania, and the other 2 strains were subdivided into two strains. The results of morphological identification of 13 samples of Oncomelania hupensis were consistent with the results of gene identification, which were similar to those of Oncomelania hupensis (87.08) and the open Oncomelania hupensis (88.55) of the new Oncomelania hupensis. The results of morphological identification of the other 12 samples were different from those of gene identification, and there were great differences between the two methods. Conclusion the subfamilies of Oncomelania hupensis can be judged accurately by COI gene detection. However, the accuracy of generic identification needs further study.
【作者单位】：厦门市疾病预防控制中心;南方医科大学公共卫生学院;福建省疾病预防控制中心;
【基金】：福建省科技厅项目(No.2011D015)~~
【分类号】：R383.2

【相似文献】