白藜芦醇对高血脂模型小鼠的预防作用和对模型金黄地鼠的改善作用研究

发布时间：2018-03-06 20:42

本文选题：白藜芦醇　切入点：高血脂　出处：《中国药房》2017年31期 　论文类型：期刊论文

【摘要】：目的:研究白藜芦醇对高血脂模型小鼠的预防作用和对模型金黄地鼠(以下简称地鼠)的改善作用。方法:以高脂高胆固醇饲料饲养诱导高血脂动物模型。将小鼠和地鼠均分为模型组和白藜芦醇低、中、高剂量组(小鼠给予40、80、160 mg/kg,地鼠给予25、50、100 mg/kg),每组10只,同时设立10只相应动物为正常对照组。除正常对照组动物喂食正常饲料和ig生理盐水外,其余小鼠在喂食高脂高胆固醇饲料的同时ig相应药物;其余地鼠先建模2周,成模后再ig相应药物,给药期间继续喂食高脂高胆固醇饲料。持续给药4周,每周检测各组小鼠和地鼠血清中总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)水平,给药4周后检测各组小鼠和地鼠血清中前蛋白转化酶枯草溶菌素9(PCSK9)mRNA及蛋白、微小RNA-27a(miRNA-27a)表达,以及小鼠肝组织中低密度脂蛋白受体(LDLR)蛋白表达。结果:与正常对照组比较,模型组小鼠从给药2周后开始血清中TC、LDL-C、PCSK9 mRNA及蛋白、miRNA-27a水平均明显升高(P0.05),肝组织中LDLR蛋白水平明显降低(P0.05);模型组地鼠给药期间血清中TC、LDLC、PCSK9 mRNA及蛋白、miRNA-27a水平均明显升高(P0.05)。与模型组比较,各给药组小鼠和地鼠上述指标均明显改善(P0.05),且与剂量呈正相关。结论:白藜芦醇对高血脂模型小鼠有预防作用,对模型地鼠有改善作用。其机制可能是通过下调血清中miRNA-27a表达与PCSK9蛋白表达,进而升高肝组织中LDLR蛋白水平,最终降低血清中LDL-C水平。
[Abstract]:Objective: to study the preventive effect of resveratrol on hyperlipidemia model mice and its improvement on golden hamster (hamster). Methods: hyperlipidemia animal model was induced by feeding high fat and high cholesterol diet. Rats and hamsters were divided into model group and low resveratrol group. In the middle and high dose group (mice were given 40,80,160 mg / kg, and the hamsters were given 2550g / kg 100 mg / kg), 10 of the corresponding animals were set up as the normal control group, except the normal control group animals were fed with normal feed and ig normal saline. The other mice were fed with high fat and high cholesterol diet, while the other mice were given corresponding drugs. The other hamsters were modeled for 2 weeks, then ig the corresponding drugs. During the course of administration, the mice continued to be fed with high fat and high cholesterol diet. The mice were given the drug for 4 weeks. Serum total cholesterol (TCC) and low density lipoprotein cholesterol (LDL-C) levels were measured weekly in mice and hamsters. After 4 weeks of administration, the mRNA and protein expression of proprotein converting enzyme 9 pPCSK9 mRNA and protein, and the expression of microRNA-27a1 miRNA-27a in serum of mice and hamsters were detected. And the expression of LDLR protein in liver tissue of mice. Results: compared with the normal control group, The serum levels of TCnLDL-CpSKPC9 mRNA and miRNA-27a in the model group increased significantly, while the level of LDLR protein in the liver tissue decreased significantly in the model group, while in the model group, the levels of TCL-LDL-CpSK9 mRNA and miRNA-27a in the serum of the model group were significantly higher than those in the model group, and compared with the model group, the levels of TCL-LDL-CpSK9 mRNA and miRNA-27a in the model group were significantly higher than those in the model group. The above indexes in each administration group and hamster were significantly improved, and were positively correlated with the dosage. Conclusion: resveratrol has preventive effect on hyperlipidemia model mice. The mechanism may be by down-regulating the expression of miRNA-27a and PCSK9 protein in serum, and then increasing the level of LDLR protein in liver tissue, and finally decreasing the level of LDL-C in serum.
【作者单位】：郑州大学附属肿瘤医院/河南省肿瘤医院药学部;郑州人民医院药学部;
【分类号】：R285.5;R-332

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