SD大鼠腹腔高压动物模型建立及肝损伤的实验研究

发布时间：2018-03-06 23:26

  本文选题：腹腔高压　切入点：腹腔压力　出处：《广西医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：建立大鼠腹腔高压（Intra-abdominal hypertension, IAH）模型，观察不同腹腔压力（Intra-abdominal pressure, IAP）及持续不同时间对肝脏的影响，为进一步研究IAH与多器官功能障碍关系作准备，为临床监测IAP的频率提供实验依据。 方法：通过往腹腔内注入氮气建立大鼠IAH模型；以一次性使用静脉输液针穿刺腹腔，连接一次性输液器，通过三通管连接血压计、氮气储气袋和50ml注射器。将45只健康雄性SD（Sprague-Dawley）大鼠，随机分为对照组（15只）和模型组（30只）。模型组按不同IAP水平分为10mmHg组和20mmHg组，该两组以IAP维持1h、2h和4h三个时间点再随机分为三个组，每组5只SD大鼠。对照组仅行腹腔穿刺置针处理，不往腹腔内注入氮气，亦随机分为1h、2h和4h组。比较模型组初次达相应IAP并稳定30s不变时，氮气注入量的变化。所有大鼠达预定实验时间点时，迅速腹主动脉采血检测血清AST、ALT的含量变化，并取肝右叶组织行组织病理学检查。 结果：1.动物模型：本研究制作的大鼠IAH模型制作简便，费用低，IAP稳定可靠，副作用少，克服了以往实验所采用的IAH动物模型的缺点。2.两模型组大鼠质量比较，差异无统计学意义（IAP10mmHg组:311.73±35.18g vs. IAP20mmHg组:295.67±19.99g，t=1.538，P＞0.05）。IAP20mmHg组大鼠腹腔内氮气注入量（146.87±14.25）ml明显大于IAP10mmHg组（127.73±12.00）ml（t=3.978，P＜0.001）。3.IAP10mmHg4h组血清AST、ALT含量分别为（297.40±48.42）U/L、（173.60±73.67）U/L，均显著高于IAP10mmHg1h组(169.40±47.69）U/L、（74.00±22.57）U/L（均P＜0.01）和IAP10mmHg2h组（223.40±61.78）U/L、（103.60±40.44）U/L（均P＜0.05）；但IAP10mmHg2h组与1h组比较，差异无统计学意义（P＞0.05）。IAP20mmHg4h组血清AST、ALT含量分别为（398.80±83.35）U/L、（237.20±74.40）U/L，均显著高于IAP20mmHg1h组（199.60±33.95）U/L、（81.40±30.53）U/L（均P＜0.01）和IAP20mmHg2h组（306.40±72.40）U/L、（154.20±42.03）U/L（均P＜0.05）；IAP20mmHg2h组血清AST、ALT含量与IAP20mmHg1h组相比，差异亦有统计学意义（均P＜0.05）。模型组IAP维持1h血清AST、ALT含量变化与对照组1h组比较，差异无统计学意义（均P＞0.05）。IAP维持2h血清AST、ALT含量变化：IAP20mmHg2h组血清AST、ALT含量分别为（306.40±72.40）U/L、（154.20±42.03）U/L，均显著高于对照组2h组（158.00±33.92）U/L、（77.60±7.16）U/L （均P＜0.01）和IAP10mmHg2h组（223.40±61.78）U/L、（103.60±40.44）U/L（均P＜0.05）。IAP维持4h血清AST、ALT含量变化：IAP20mmHg4h组血清AST、ALT含量分别为（398.80±83.35）U/L、（237.20±74.40）U/L，与对照组4h组（160.80±21.30）U/L、（75.20±18.43）U/L比较，差异具有统计学意义（均P＜0.01），与IAP10mmHg4h组血清AST（297.40±48.42）U/L比较，差异亦有统计学意义（P＜0.05），但与IAP10mmHg4h组血清ALT（173.60±73.67）U/L比较，差异无统计学意义（P＞0.05）；IAP10mmHg4h组血清AST、ALT含量与对照组4h组比较，差异具有统计学意义（P＜0.01，P＜0.05）。4.肝组织病理形态学变化：IAP10mmHg4h组中央静脉周边少许肝细胞轻度浊肿变性，可见空泡变性，核肿胀，近中央静脉区肝窦轻度扩张、充血。IAP20mmHg1h组，肝小叶结构清楚，中央静脉周边少许肝细胞轻度浊肿变性，排列稍紊乱，细胞边界欠清晰，肝窦轻度充血；IAP20mmHg组随IAP持续时间延长肝细胞损伤范围增大。IAP20mmHg4h组，肝小叶结构紊乱，可见肝细胞点状坏死，少许炎症细胞浸润。 结论：1.成功制作了稳定可靠的大鼠IAH动物模型，，本IAH模型制作、操作简单，耗费低，容易复制，对肝功能损伤效果明显。2.IAP达一定程度时，较小的腹腔内容积变化亦可引起较大的IAP变化。3.IAH可导致肝损伤，同一IAP，IAP持续时间越长，肝损伤越严重；同一作用时间，IAP越高，肝损伤越重。IAP10mmHg作用4h中央静脉周边少许肝细胞轻度水肿；IAP20mmHg作用1h可能已有轻度肝损伤，IAP20mmHg作用4h，可见肝细胞点状坏死，少许炎症细胞浸润。
[Abstract]:Objective: to establish a rat model of intra-abdominal hypertension (Intra-abdominal, hypertension, IAH) model, to observe the effects of different intra-abdominal pressure (Intra-abdominal pressure, IAP) and the influence of different duration on the liver, preparing for the further study of the relationship between IAH and multiple organ dysfunction, and provide experimental basis for clinical monitoring of IAP frequency.
Methods: by intraperitoneal injection of gaseousnitrogen rat IAH model; the disposable intravenous infusion needle was connected with a disposable transfusion device, connected with a sphygmomanometer through three way pipe, nitrogen gas storage bag and 50ml syringe. A total of 45 healthy male SD (Sprague-Dawley) rats were randomly divided into control group (15) and the model group (30 rats). The model group according to different levels of IAP were divided into 10mmHg group and 20mmHg group, the two groups to maintain IAP 1H, 2h and 4H three time points and then randomly divided into three groups, each group had 5 SD rats. The control group was treated with abdominal puncture needle treatment, not to intraperitoneal injection of nitrogen, were also randomly divided into 1H, 2h and 4H group. The first group of IAP and the corresponding model is stable 30s unchanged, changes in the amount of nitrogen injection. All rats reached a predetermined experimental time point, rapid detection of serum AST abdominal aorta blood and the content of ALT, and the right lobe of the liver tissue histopathological Study.
Results: 1. animal model: the IAH rat model made of simple production, low cost, IAP is stable and reliable, less side effects, to overcome the disadvantages of IAH.2. quality group rats animal model was used in the experiments of the two models, the difference was not statistically significant (IAP10mmHg group: IAP20mmHg group: vs. 311.73 + 35.18g 295.67 + 19.99g, t=1.538, P > 0.05).IAP20mmHg rats intraperitoneal injection of nitrogen (146.87 + 14.25) ml was significantly higher than that of IAP10mmHg group (127.73 + 12) ml (t=3.978, P < 0.001) in.3.IAP10mmHg4h group serum AST, ALT were (297.40 + 48.42) U/L, (173.60 + 73.67) U/L, significantly higher than that of group IAP10mmHg1h (169.40 + 47.69) U/L, (74 + 22.57) U/L (P < 0.01) and IAP10mmHg2h group (223.40 + 61.78) U/L, (103.60 + 40.44) U/L (P < 0.05); but the IAP10mmHg2h group compared with the 1H group, there was no statistically significant difference (P > 0.05).IAP20mmHg4h group, serum AST, ALT 鍚�閲忓垎鍒�涓

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