延边地区朝鲜族和汉族男性人群六个Y染色体STR基因座的遗传多态性

发布时间：2018-03-07 05:18

本文选题：Y染色体　切入点：短串联重复序列(STR)　出处：《延边大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的：探讨Y染色体短串联重复序列(STR)基因座DYS441、DYS442、DYS443、OY3444、DYS445、DYS446的遗传多态性在延边地区朝鲜族和汉族男性人群中的分布情况。方法：(1)收集205名无血缘关系的朝鲜族男性和207名汉族男性个体的抗凝血,用血样基因组DNA提取试剂盒提取DNA。(2)应用聚合酶链式反应(PCR)、变性聚丙烯酰胺凝胶电泳和银染显带技术对DYS441～DYS446六个基因座进行检测。(3)对各基因座的等位基因进行测序,制备等位基因分型标准物,并根据国际法医遗传学会DNA委员会(ISFG DNA Commission)推荐原则命名等位基因。(4)收集数据并进行统计学分析。结果：DYS441、DYS442、DYS443、DYS444、DYS445、DYS446基因座,在朝鲜族男性人群中依次检测出8、7、7、5、6、9个等位基因,其遗传多样性(gene diversity GD)依次为0.7493、0.5083、0.6280、0.6969、0.5302、0.7897,联合6个Y-STR基因座分析,共检测到152种不同的单倍型,单倍型变异度为0.9501。汉族男性依次检测出8、5、6、6、5、10个等位基因,其遗传多样性(gene diversity GD)依次为0.7493、0.5083、0.6280、0.6969、0.5302、0.7897,联合6个Y-STR基因座分析,共检测到174种不同的单倍型,单倍型变异度为0.9880。结论：DYS441、DYS442、DYS443、DYS444、DYS445、DYS446基因座有较高的遗传多态性和稳定性,所得到的等位基因频率数据可为延边地区朝鲜族和汉族人群的法医学个人识别、亲权鉴定、民族遗传学研究提供遗传多态性依据。
[Abstract]:Objective: to investigate the genetic polymorphism of DYS441DYS442DYS443OY3444 (DYS444) and DYS445DYS446 in male population of Korean and Han nationality in Yanbian area, and to investigate the distribution of DYS441DYS442DYS443OY3444 and DYS445DYS446 in male population of Korean and Han nationality in Yanbian area. Methods the anticoagulant blood samples were collected from 205 unrelated Korean males and 207 Han males. The alleles of DYS441~DYS446 were sequenced by polymerase chain reaction (PCR), denatured polyacrylamide gel electrophoresis (DPAGE) and silver staining banding technique. The allelic alleles were prepared and named according to the principle recommended by the DNA DNA Commission of the International Society of Forensic Genetics. Results: DYS441DYS442DYS442DYS443DYS444-DYS445DYS446 loci were detected in order of 6alleles and 9 alleles in Korean male population. The genetic diversity of DYS442DYS442DYS442DYS442DYS442DYS442DYS442DYS442DYS442DYS442DYS442DYS442DYS442DYS442DYS4442DYS445DYS446 alleles and 9 alleles were sequentially detected in the Korean male population. The genetic diversity of diversity GDs was 0.74930.50830.6280f0. The variation of haplotype was 0.9501.The genetic diversity gene diversity GDwas 0.74930.5083n0.62800.9699 and 0.53020.7897respectively, and 174 different haplotypes were detected in combination with 6 Y-STR loci. The variation of haplotypes was 0.98800.The variation of haplotypes was 0.98800.The results showed that the genetic diversity of haplotypes was 0.98800.9880, and the genetic diversity was 0.7493C (0.5083), 0.62800.9699 (0.5302P) 0.78977.Combined with 6 Y-STR loci, 174 haplotypes were detected. Conclusion the genetic polymorphism and stability of DYS441 / DYS442 / DYS443 / DYS444 / DYS444 / DYS44446 loci are higher than those of DYS441 / DYS441 / DYS442DYS442 / DYS443 / DYS444 / DYS444 / DYS444 / DYS44446 loci. The study of ethnic genetics provides the basis for genetic polymorphism.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R394

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