探讨染色体Cd结构及CENP变化与非整倍体形成的关系

发布时间：2018-03-07 09:43

本文选题：染色体　切入点：非整倍体　出处：《山西医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：研究流产绒毛染色体非整倍体及其双亲染色体着丝粒点(Cd)结构变异和着丝粒蛋白(CENP)表达情况，探讨子代非整倍体形成及其双亲染色体Cd结构变异和CENP表达的关系。方法：以非整倍体流产绒毛及其双亲为研究组，以正常绒毛及其双亲为对照组。应用染色体G显带技术、改良的着丝粒点-核仁组织区(Cd - NOR)同步银染方法及免疫组化技术，对每例标本进行染色体核型分析、Cd变异及CENP检测。结果：①两组流产绒毛染色体的Cd变异有显著差异，研究组中的流产绒毛染色体Cd变异明显高于对照组（t＝14.785，p㩳0.05）。②研究组中的流产绒毛染色体Cd变异高于双亲外周血染色体，差异有统计学意义,（t＝②14.965 p②㩳0.05，t＝③15.371，p③㩳0.05）。③对照组中的流产绒毛染色体Cd变异与其双亲外周血染色体比较，差异无统计学意义（t④＝2.463 p④㧐0.05，t＝⑤2.156 p⑤㧐0.05）④两组流产绒毛染色体中均有CENP的表达，且研究组(灰度值：165.53±4.81)显著低于对照组(灰度值：160.87±4.62)( t＝3.012，p㩳0.05)。⑤研究组非整倍体绒毛染色体中CENP含量低于其父方，差异有统计学意义（t①＝-4.279，p①㩳0.05），而与母方的差异无统计学意义（t②＝-1.927,p②㧐0.05)。⑥对照组正常绒毛染色体CENP含量低于其父方，差异有统计学意义（t③＝-3.008,p③㩳0.05),而与母方的差异无统计学意义(t④＝-1.234 p④㧐0.05)。⑦CENP的含量与Cd结构变异呈负相关。结论：Cd变异、CENP含量降低可能是染色体着丝粒不分离造成非整体形成的原因之一，且CENP的含量与其母亲具有一定的同源性，染色体中CENP的含量与Cd变异呈负相关，即Cd变异数/细胞数越高，CENP的含量越少。
[Abstract]:Objective: to study the structural variation of chromosomal aneuploidy (CDD) and the expression of centromere protein (CENPP) in chromosomal aneuploidy of abortive villi and their parents. To investigate the relationship between the formation of aneuploidy and the relationship between CD structural variation and CENP expression in the chromosomes of their parents. Methods: using aneuploidy abortion villi and their parents as study group and normal villi and their parents as control group, chromosome G banding technique was used. The modified Cd-NORs simultaneous silver staining method and immunohistochemical technique were used to analyze the chromosome karyotype variation and CENP. Results there were significant differences between the two groups of abortive villi. The chromosome CD variation of abortive villi in the study group was significantly higher than that in the control group. The CD variation of abortive villi chromosomes in the study group was higher than that in the peripheral blood chromosomes of both parents, and the difference was statistically significant. 0.05t ~ (315.371n) p ~ (3)? The chromosome CD variation of abortive villi in the control group was not significantly different from that of their parents' peripheral blood chromosomes. 0. 05 TX 52.156 p5? The expression of CENP in the villus chromosomes of both groups was significantly lower in the study group (gray value: 165.53 卤4.81) than that in the control group (gray value: 1: 160.87 卤4.62). The CENP content in the chromosomes of aneuploidy villi in the study group was lower than that in its father's side, and the difference was statistically significant. There was no statistical difference between the two groups and the difference between them was not statistically significant (t _ (2)) -1.927 (P ~ (2))? The CENP content of normal villi in the control group was lower than that of its father's side, and the difference was statistically significant. 0. 05%, but there was no statistical difference between the two groups. There was a negative correlation between the content of 0. 05 and 7 CENP and the variation of CD structure. Conclusion the decrease in the content of CENs in the chromosome of 1: CD mutation may be one of the reasons for the formation of nonwhole in chromosome centromere dissection, and the content of CENP has some homology with its mother, and the content of CENP in chromosome is negatively correlated with the variation of CD. That is, the higher the number of CD variation / cell number, the less the content of CENP.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R394

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