嗅觉损伤诱导触须触觉跨模式可塑性的细胞学基础研究

发布时间：2018-03-08 08:34

本文选题：桶状皮层　切入点：跨感觉可塑性　出处：《蚌埠医学院》2011年硕士论文　论文类型：学位论文

【摘要】：目的：本研究旨在探讨小鼠嗅觉损伤后触觉跨模式可塑性桶状皮层的细胞机理，尤其是比较长短触须触觉代表区A5-E1和C9-E4内锥体神经元可塑性的差异。方法：1.行为学实验：选用出生后12天的小鼠，建立嗅觉剥夺诱导触觉功能上调的动物模型，实验分为两组，正常组和剥夺组。嗅觉剥夺后1周到2周，应用数码照相机对小鼠行为学进行视频拍摄，然后在视频软件中记录单位时间内（30s）长、短触须摆动的次数(频率)及触须屈曲的时间。 2.膜片钳电生理实验：在嗅觉剥夺后1周，用异氟烷吸入麻醉，迅速将大脑分离，将大脑桶状皮层分为与短触须对应的C9-E4区和与长触须对应的A5-E1区，然后将大脑分离并做冠状切片(400μm)，，分别将C9-E4区和A5-E1区的脑片放置于氧合的人工脑脊液(ASCF)中，25C人工脑脊液中，孵育1-2小时后转移至灌流槽内，在31C下对脑片进行灌流；采用IR-DIC光学显微镜(Nikon E600FN)荧光显微镜定位大脑桶状皮层锥体神经元；运用Axoclamp-200B放大器全细胞记录大脑桶状皮层锥体神经元的输入输出曲线和桶状皮层C9-E4区和A5-E1区锥体神经元动作电位的阈电位(threshold potentials,Vts)、峰电位间距，信号输入Clampex10.2软件获取群集发放的动作电位间距(inter-spike interval,ISI)，使用Clampfit10.2软件进行数据分析。结果：1.嗅觉剥夺小鼠触须较正常组摆动频率增加，剥夺组的长触须摆动频率较短触须更显著(P0.05)；2.应用膜片钳电生理方法，较正常组相比，剥夺组桶状皮层锥体神经元发放功能增强(P0.05)。尤其是在长触须代表区的A5-E1的桶状皮层功能上调更显著(P0.05)。结论：1.嗅觉损伤诱导的触觉跨模式可塑性的研究中，桶状皮层锥体神经元的功能上调。2.嗅觉、触觉存在的跨感觉可塑性和桶状皮层神经元功能上调在与长触须代表区A5-E1表现更显著。
[Abstract]:Aim: to investigate the cellular mechanism of the transmodal plasticity bucket cortex of tactile sensation after olfactory injury in mice, especially to compare the difference of the plasticity of pyramidal neurons between A5-E1 and C9-E4 in the tactile representative regions of the long and short tentacles. Methods 1. Behavioral experiment: mice were selected 12 days after birth to establish an animal model of increased tactile function induced by olfactory deprivation. The experiment was divided into two groups: normal group and deprivation group, 1 week to 2 weeks after olfactory deprivation. The mouse behavior was photographed by digital camera, and the number of times (frequency) of wobble of tentacles and the time of tentacles flexion were recorded in the video software in a unit time of 30 s. 2. Patch clamp electrophysiological experiment: 1 week after olfactory deprivation, isoflurane was inhaled to anesthetize the brain, and the brain barrel cortex was divided into C9-E4 region corresponding to short tentacles and A5-E1 region corresponding to long tentacles. Then the brain was separated and coronal sliced with 400 渭 m. The brain slices of C9-E4 area and A5-E1 area were placed in 25 C artificial cerebrospinal fluid in oxygenated artificial cerebrospinal fluid (ASCF), incubated for 1-2 hours, then transferred to the perfusion trough. The slices were perfused at 31C. The pyramidal neurons in the barrelled cortex were located with IR-DIC optical microscope and Nikon E600FN fluorescence microscope. The input and output curves of the pyramidal neurons in the barrelled cortex and the threshold potentials of the action potentials of the pyramidal neurons in the C9-E4 and A5-E1 regions of the brain were recorded by Axoclamp-200B amplifiers. The signal input Clampex10.2 software was used to obtain the inter-spike interval between the action potentials issued by the cluster, and the data were analyzed using Clampfit10.2 software. Results 1. The frequency of wobble of tentacles of olfactory deprived mice was higher than that of normal group, and the frequency of wobble of long tentacles in deprivation group was more significant than that of short tentacles. 2. Using patch clamp electrophysiological method, compared with normal group, In deprivation group, the function of pyramidal neurons in bucket cortex was enhanced (P 0.05), especially in the area of long tentacular representative area (A5-E1), the function of bucket cortex was upregulated more significantly (P 0.05). Conclusion 1. In the study of transmodal plasticity of tactile neurons induced by olfactory injury, the function of pyramidal neurons in bucket cortex is up-regulated. The transsensory plasticity of tactile and the up-regulation of the function of bucket cortical neurons were more significant in A5-E1 with long tentacles.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R338.1

【参考文献】