免疫抑制剂对巨噬细胞Dectin-1和TLR2表达的影响

发布时间：2018-03-09 18:20

本文选题：免疫抑制剂　切入点：Dectin-　出处：《中国免疫学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:研究地塞米松、环磷酰胺、环孢素A和霉酚酸酯对小鼠RAW264.7巨噬细胞Dectin-1和TLR2受体表达的影响。方法:体外培养RAW264.7巨噬细胞,分别给予不同浓度的地塞米松、环磷酰胺、环孢素A和霉酚酸酯刺激细胞24 h,CCK-8检测RAW264.7细胞毒性作用,RT-PCR检测细胞Dectin-1和TLR2 mRNA表达情况,流式细胞术检测Dectin-1和TLR2蛋白水平变化。结果:不同剂量的地塞米松、环磷酰胺、环孢素A和霉酚酸酯作用细胞24 h后均对细胞表现出一定的毒性作用(P0.05),且随着药物浓度增加毒性作用越大。地塞米松降低Dectin-1 mRNA和蛋白水平的表达,上调TLR2受体转录和翻译(P0.05);环磷酰胺对巨噬细胞Dectin-1和TLR2的转录翻译无明显影响(P0.05);霉酚酸酯和环孢素A能够同时下调Dectin-1、TLR2 mRNA和蛋白水平表达(P0.05)。结论:不同免疫抑制剂对模式识别受体表达的调节作用具有差异性,通过选择性调节各自靶点PRRs的表达抑制机体对各种真菌病原体的免疫识别过程,同时也能够抑制巨噬细胞的生长增殖,共同介导免疫抑制功能。
[Abstract]:Objective: to study the effects of dexamethasone, cyclophosphamide, cyclosporine A and mycophenolate mofetil on the expression of Dectin-1 and TLR2 receptors in mouse RAW264.7 macrophages. Cyclosporine A and mycophenolate mofetil stimulated by CCK-8 were used to detect the cytotoxicity of RAW264.7 cells. RT-PCR was used to detect the expression of Dectin-1 and TLR2 mRNA, and flow cytometry was used to detect the changes of Dectin-1 and TLR2 protein levels. Results: different doses of dexamethasone, cyclophosphamide and cyclophosphamide were detected. Cyclosporine A and mycophenolate mofetil had a certain toxic effect on the cells 24 h later, and the more toxic effect was with the increase of drug concentration, dexamethasone decreased the expression of Dectin-1 mRNA and protein. TLR2 receptor transcription and translation was up-regulated (P0.05A); cyclophosphamide had no effect on Dectin-1 and TLR2 transcriptional translation of macrophages; mycophenolate mofetil and cyclosporine A could down-regulate the expression of Dectin-1TLR2 mRNA and protein at the same time. Conclusion: different immunosuppressants have a positive effect on the expression of TLR2 mRNA and protein in macrophages. The regulatory effects of pattern recognition receptor expression are different. By selectively regulating the expression of PRRs, we can inhibit the immune recognition process of various fungal pathogens, but also inhibit the growth and proliferation of macrophages, and jointly mediate the immunosuppressive function.
【作者单位】：安徽医科大学;空军总医院呼吸内科;
【分类号】：R392

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