干细胞拉曼光谱收集条件选择及间充质干细胞与胚胎干细胞拉曼光谱的比较

发布时间：2018-03-10 01:13

本文选题：间质干细胞　切入点：胚胎干细胞　出处：《中国组织工程研究》2017年17期 　论文类型：期刊论文

【摘要】：背景:拉曼光谱在检测细胞状态方面相比于传统技术具有快速、非介入、非标记等特点,因此在生物医学领域中的应用正日益受到关注,但是该技术应用于干细胞检测的条件还有待进一步优化。目的:探索激光波长与光栅刻度密度对于干细胞拉曼光谱分辨率与收集时间等参数的影响,明确可以用于干细胞分析的拉曼光谱收集条件。方法:首先比较532,638,785 nm 3个波长激光及600,1 200,1 800 gr/mm 3种光栅刻度密度条件对人间充质干细胞与人胚胎干细胞拉曼光谱的影响;之后比较这2个条件不同组合得到的拉曼光谱的光谱分辨率与收集时间,选择出最佳的组合条件;最后使用选出的最佳条件结合主成分分析方法比较胚胎干细胞与间充质干细胞拉曼光谱的差异以验证优化条件的适用性。结果与结论:(1)激光波长与光栅刻度密度均会不同程度影响干细胞拉曼光谱的峰强,进而影响特征性拉曼峰之间的比值;(2)785 nm波长激光与1 200 gr/mm光栅刻度密度条件组合是最适合收集条件;(3)对比分析胚胎干细胞与间充质干细胞的拉曼光谱图,提示胚胎干细胞具有更高的核酸含量,而间充质干细胞在一些蛋白和脂类含量上更为丰富。
[Abstract]:Background: compared with traditional techniques, Raman spectroscopy has many advantages, such as rapid, non-interventional and non-labeling, so its application in biomedical field has attracted more and more attention. But the conditions for the application of this technique to stem cell detection need to be further optimized. Objective: to explore the effects of laser wavelength and grating calibration density on the Raman spectral resolution and collection time of stem cells. Methods: the effects of three wavelength laser at 532,638,785 nm and 1 800 gr/mm on the Raman spectra of human mesenchymal stem cells and human embryonic stem cells were compared. Then the spectral resolution and collection time of Raman spectra obtained by different combinations of these two conditions were compared and the optimal combination conditions were selected. Finally, the difference of Raman spectra between embryonic stem cells and mesenchymal stem cells was compared using the selected optimal conditions and principal component analysis to verify the applicability of the optimized conditions. Results and conclusion: laser wavelength and grating calibration density. The peak intensity of Raman spectra of stem cells is affected by different degrees. Furthermore, the ratio of characteristic Raman peaks is affected by the combination of wavelength laser and 1 200 gr/mm grating calibration density. The Raman spectra of embryonic stem cells and mesenchymal stem cells are compared and analyzed, which is the most suitable condition for collecting the Raman spectra of embryonic stem cells and mesenchymal stem cells (mesenchymal stem cells). These results suggest that embryonic stem cells have higher nucleic acid content, while mesenchymal stem cells are more abundant in some proteins and lipids.
【作者单位】：中国医学科学院北京协和医学院血液学研究所血液病医院实验血液学国家重点实验室;
【基金】：国家863高技术研究发展计划(2011AA020118) 国家973重点基础项目(2012CB966504)~~
【分类号】：R329.2

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