促红细胞生成素对速发型哮喘小鼠模型IL-4、IL-5及IgE指标的影响

发布时间：2018-03-12 17:08

本文选题：促红细胞生成素　切入点：速发型哮喘　出处：《毒理学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的探讨促红细胞生成素(EPO)对速发型哮喘小鼠模型IL-4、IL-5及IgE的变化及肺组织病理变化的相关机制影响。方法选择54只Balb/c小鼠随机分为6组,每组9只。哮喘模型组,EPO高、中、低浓度组和地塞米松组,每间隔7 d腹腔注射20μg卵清蛋白(ovalbumin,OVA)+2 mg氢氧化铝共0.2 ml致敏3次。第22天引喘小鼠,用3%OVA溶液(生理盐水配制)雾化吸入,吸入30 min/d,持续7 d。EPO低、中、高浓度组和地塞米松组分别于雾化吸入前30 min腹腔注射低浓度EPO(500 IU/kg)、中浓度EPO(1 000 IU/kg)、高浓度EPO(2 000 IU/kg)和地塞米松(1 mg/kg)。正常对照组腹腔注射和雾化吸入的时间和方法相同只是使用生理盐水替代药品。通过酶联免疫吸附实验法(ELISA)检测血清中IL-5、IL-4和IgE水平;小鼠肺组织行切片,苏木素-伊红(HE)染色,观察其病理变化。结果EPO高、中、低浓度组,哮喘模型组和地塞米松组IL-4、IL-5及IgE浓度均高于正常对照组,差异有统计学意义(P0.05)。EPO高、中浓度组和地塞米松组IL-4、IL-5及IgE浓度均低于哮喘模型组,差异有统计学意义(P0.05)。正常对照组中小鼠支气管的上皮无损伤,炎症反应表现不明显,且肺组织形态无异常;而哮喘模型组中小鼠存在很多的炎性细胞,其中大部分存在于支气管及其周围血管,最终致使管腔狭窄;EPO低浓度组结果显示存在的炎性细胞及管腔狭窄程度较哮喘组减轻,随EPO浓度的增加,病理损伤程度减轻;EPO高、中和低浓度组与地塞米松组比较,管腔及平滑肌厚度相似,但地塞米松组炎性细胞浸润程度明显减轻。结论 EPO可抑制哮喘小鼠IL-4、IL-5、IgE的分泌及减轻肺组织病理改变,提示EPO对哮喘小鼠模型的炎症因子的产生具有调节作用。
[Abstract]:Objective to investigate the effects of erythropoietin (EPO) on the changes of IL-4 IL-5 and IgE and the pathological changes of lung tissue in mouse model of acute asthma. Methods 54 Balb/c mice were randomly divided into 6 groups with 9 in each group. In the low concentration group and dexamethasone group, 20 渭 g ovalvalbumin OVA (20 渭 g ovalbumin OVA) 2 mg aluminum hydroxide was injected intraperitoneally every 7 days for 3 times. The high concentration group and dexamethasone group were injected intraperitoneally with low concentration of EPO(500 / kg, moderate concentration of EPO(1 / kg, high concentration of EPO(2 / kg / kg) and dexamethasone 1 mg / kg respectively at 30 min before atomization inhalation. The time and method of intraperitoneal injection and inhalation of dexamethasone were the same in normal control group. The serum levels of IL-5 IL-4 and IgE were detected by Elisa. Results the levels of IL-4 IL-5 and IgE in the high, middle and low EPO groups, asthma model group and dexamethasone group were higher than those in the normal control group, and the difference was statistically significant. The concentrations of IL-4 IL-5 and IgE in the middle concentration group and dexamethasone group were lower than those in the asthmatic model group (P 0.05). However, there were many inflammatory cells in the asthmatic model group, most of which were found in the bronchi and its surrounding blood vessels, which resulted in the lower concentration of EPO in the lumen stenosis and the degree of the inflammatory cells and the stenosis of the lumen were less than those in the asthmatic group. With the increase of EPO concentration, the degree of pathological injury was reduced. The thickness of lumen and smooth muscle was similar in medium and low concentration groups compared with dexamethasone group. Conclusion EPO can inhibit the secretion of IL-4 IL-5 IgE and alleviate the pathological changes of lung tissue in asthmatic mice, suggesting that EPO can regulate the production of inflammatory factors in asthmatic mice.
【作者单位】：延边大学附属医院;
【分类号】：R-332;R562.25

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