刚地弓形虫棒状体蛋白10的生物信息学分析

发布时间：2018-03-12 21:02

本文选题：刚地弓形虫　切入点：TgROP　出处：《中国病原生物学杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的对刚地弓形虫棒状体蛋白10(TgROP10)进行生物信息学预测,分析其免疫原性。方法运用生物信息学在线分析程序ProtParam、SOSUI、ProtScale、TMHMM、SignalP-4.1、TargetP1.1Server、SOPMA、MotifScan、SWISS-MODEL、SYFPEITHI和Bcepred等并结合生物信息学软件DNASTAR和DNAMAN分析预测TgROP10的理化性质、可溶性、亲疏水性、跨膜结构域、信号肽、胞内定位、二级结构、蛋白翻译后修饰位点、三级结构、柔韧性、表面可及性和B细胞、T细胞抗原表位等。结果 TgROP10蛋白由586个氨基酸组成,分子式为C2562H4045N739O964S9,分子质量单位(Mr)为60.912 21×103,理论等电点为4.19,吸光度值为0.476,半衰期为30h,不稳定系数为55.56,脂溶性指数为56.25,总平均亲水性系数为-0.699,属于亲水性蛋白;该蛋白无跨膜结构区域,信号肽切割位点在22-23位氨基酸之间,亚细胞定位预测可能是分泌蛋白;二级结构中α螺旋和β折叠分别占26.79%和13.31%,β转角和无规则卷曲分别占6.48%和53.41%,有6种翻译后修饰位点,15个B细胞抗原表位,3个CTL细胞抗原表位,12个Th细胞抗原表位,28个亲水性参数得分1.9的区域,15个柔韧性参数得分2.0的区域,27个表面可及性参数得分1.9的区域,3个暴露表面参数得分2.3的区域。结论生物信息学预测TgROP10含多个抗原表位,具有免疫原性,可作为弓形虫疫苗备选蛋白。
[Abstract]:Objective to predict and analyze the immunogenicity of Toxoplasma gondii (TGROP10) by bioinformatics. Methods the physicochemical properties of Toxoplasma gondii were predicted by using the bioinformatics online analysis program ProtParamia so sui. TMHMMMM-SignalP-1.1 TargetP1.1 Server SOPMAMotifScanSWISS-MODELS-MOFPEITHI and Bcepred, combined with bioinformatics software DNASTAR and DNAMAN to predict the physical and chemical properties of TgROP10. Soluble, hydrophobic, transmembrane domain, signal peptide, intracellular localization, secondary structure, protein post-translational modification site, tertiary structure, flexibility, Results the TgROP10 protein was composed of 586 amino acids. The molecular formula is C2562H4045N739O964S9, the unit of molecular weight is 60.91221 脳 10 ~ 3, the theoretical isoelectric point is 4.19, the absorbance is 0.476, the half-life is 30 h, the instability coefficient is 55.56, the lipid solubility index is 56.25, the total average hydrophilic coefficient is -0.699, which belongs to hydrophilic protein. The signal peptide cleavage site is between 22 and 23 amino acids, and the subcellular localization may be secretory protein. 伪 helix and 尾 folding accounted for 26.79% and 13.31 in the secondary structure, 6.48% and 53.41 in 尾 rotation and irregular crimp, respectively. There were 6 posttranslational modified sites, 15 B cell antigen epitopes, 3 CTL cell antigen epitopes and 12 Th cell antigen epitopes. There were 1. 9 areas with hydrophilic parameter score, 15 areas with flexibility parameter score 2. 0, 27 areas with surface accessibility parameter 1. 9 and 3 areas with exposure surface parameter score 2. 3. Conclusion Bioinformatics predicts that TgROP10 contains multiple antigenic epitopes. It has immunogenicity and can be used as an alternative protein for Toxoplasma gondii vaccine.
【作者单位】：河北北方学院病原生物学与免疫学研究所;
【基金】：河北省自然科学基金项目(No.H2013405091) 河北北方学院校级重大课题(No.ZD201312)
【分类号】：R382.5

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